HnRNP L-mediated regulation of mammalian alternative splicing by interference with splice site recognition
文献类型：期刊论文
| 作者 | Heiner, M; Hui, JY; Schreiner, S; Hung, LH; Bindereif, A |
| 刊名 | RNA BIOLOGY
 |
| 出版日期 | 2010 |
| 卷号 | 7期号:1页码:56-64 |
| 关键词 | splicing alternative splicing silencer hnRNP snRNP |
| 通讯作者 | Bindereif, A (reprint author), Univ Giessen, Inst Biochem, Giessen, Germany.,albrecht.bindereif@chemie.bio.uni-giessen.de |
| 英文摘要 | Heterogeneous nuclear ribonucleoprotein (hnRNP) L can regulate alternative mRNA splicing in diverse ways, binding to exonic or intronic sites and acting as either an activator or repressor. To investigate the mechanistic basis of hnRNP L-regulated alternative splicing, we focus here on two specific cases of hnRNP L-dependent splice site recognition. First, in the case of TJP1 our microarray data had suggested that exon 20 inclusion is regulated by hnRNP L as a repressor. Here we demonstrate by mutational analysis that exon skipping is mediated by a short silencer sequence consisting of three hnRNP L high-score binding motifs located upstream of the 3' splice site of the regulated exon. UV crosslinking and immunoprecipitation experiments showed that hnRNP L binding interferes with 3' splice site recognition by U2AF65. Second, SLC2A2 contains a CA-repeat sequence close to the 5' splice site of the regulated exon 4. Using psoralen crosslinking, we demonstrate that hnRNP L represses splicing by preventing 5' splice site recognition of the U1 snRNP. In sum, our data provide new insights into the mechanisms of how hnRNP L-bound to intronic sites-regulates exon recognition. |
| 学科主题 | Biochemistry & Molecular Biology |
| 类目[WOS] | Biochemistry & Molecular Biology |
| 关键词[WOS] | TRACT BINDING-PROTEIN ; PRE-MESSENGER-RNA ; POLYPYRIMIDINE TRACT ; CA REPEATS ; PREMESSENGER RNA ; PTB ; EXPRESSION ; REPRESSION ; SEQUENCE ; U2AF |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000276197800008 |
| 版本 | 出版稿 |
| 源URL | [http://202.127.25.143/handle/331003/940]  |
| 专题 | 上海生化细胞研究所_上海生科院生化细胞研究所 |
| 推荐引用方式 GB/T 7714 | Heiner, M,Hui, JY,Schreiner, S,et al. HnRNP L-mediated regulation of mammalian alternative splicing by interference with splice site recognition[J]. RNA BIOLOGY,2010,7(1):56-64. |
| APA | Heiner, M,Hui, JY,Schreiner, S,Hung, LH,&Bindereif, A.(2010).HnRNP L-mediated regulation of mammalian alternative splicing by interference with splice site recognition.RNA BIOLOGY,7(1),56-64. |
| MLA | Heiner, M,et al."HnRNP L-mediated regulation of mammalian alternative splicing by interference with splice site recognition".RNA BIOLOGY 7.1(2010):56-64. |
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