Purification and characterization of Stn1p, a single-stranded telomeric DNA binding protein
文献类型：期刊论文
| 作者 | Qian, W; Fu, XH; Zhou, JQ |
| 刊名 | PROTEIN EXPRESSION AND PURIFICATION
 |
| 出版日期 | 2010 |
| 卷号 | 73期号:2页码:107-112 |
| 关键词 | Stn1p Ten1p Telomere DNA binding Co-fractionation |
| 通讯作者 | Qian, W (reprint author), Nantong Univ, Sch Med, Dept Biochem, Nantong 226001, Jiangsu, Peoples R China.,weiqianhuang@hotmail.com |
| 英文摘要 | In Saccharomyces cerevisiae, Stn1p and Ten1p are required for telomere maintenance. These two proteins and another telomeric single-stranded DNA binding protein, Cdc13p, have been proposed to form a complex to control telomere integrity. In this work, we purified the recombinant stn1p in Escherichia coli and found that the purified protein could specifically interact with single-stranded telomeric DNA in vitro. Co-fractionation of co-overexpressed stn1p and Ten1p in insect cells revealed their stable association. A stn1p/Ten1p binary complex was reconstituted with purified recombinant proteins in vitro. These results indicated that stn1p and Ten1p interact with each other directly, which is important in telomere length regulation and end protection. (C) 2010 Elsevier Inc. All rights reserved. |
| 学科主题 | Biochemistry & Molecular Biology; Biotechnology & Applied Microbiology |
| 类目[WOS] | Biochemical Research Methods ; Biochemistry & Molecular Biology ; Biotechnology & Applied Microbiology |
| 关键词[WOS] | SACCHAROMYCES-CEREVISIAE ; LENGTH REGULATION ; END PROTECTION ; POLYMERASE-ALPHA ; CDC13 PROTEIN ; IN-VIVO ; REPLICATION ; EST1 ; ASSOCIATION ; STN1-TEN1 |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000280439000001 |
| 版本 | 出版稿 |
| 源URL | [http://202.127.25.143/handle/331003/944]  |
| 专题 | 上海生化细胞研究所_上海生科院生化细胞研究所 |
| 推荐引用方式 GB/T 7714 | Qian, W,Fu, XH,Zhou, JQ. Purification and characterization of Stn1p, a single-stranded telomeric DNA binding protein[J]. PROTEIN EXPRESSION AND PURIFICATION,2010,73(2):107-112. |
| APA | Qian, W,Fu, XH,&Zhou, JQ.(2010).Purification and characterization of Stn1p, a single-stranded telomeric DNA binding protein.PROTEIN EXPRESSION AND PURIFICATION,73(2),107-112. |
| MLA | Qian, W,et al."Purification and characterization of Stn1p, a single-stranded telomeric DNA binding protein".PROTEIN EXPRESSION AND PURIFICATION 73.2(2010):107-112. |
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