Liver Xeno-Repopulation with Human Hepatocytes in Fah(-/-) Rag2(-/-) Mice after Pharmacological Immunosuppression
文献类型：期刊论文
| 作者 | He, ZY; Zhang, HB; Zhang, X; Xie, DF; Chen, YX; Wangensteen, KJ; Ekker, SC; Firpo, M; Liu, CC; Xiang, D |
| 刊名 | AMERICAN JOURNAL OF PATHOLOGY
 |
| 出版日期 | 2010 |
| 卷号 | 177期号:3页码:1311-1319 |
| 通讯作者 | Wang, X (reprint author), Chinese Acad Sci, Inst Biochem & Cell Biol, Mol Cell Biol Lab, 320 Yueyang Rd, Shanghai 200031, Peoples R China., |
| 英文摘要 | Functional human hepatocytes xeno-engrafted in mouse liver can be used as a model system to study hepatitis virus infection and vaccine efficacy. Significant liver xeno-repopulation has been reported in two kinds of genetically modified mice that have both immune deficiency and liver injury-induced donor hepatocyte selection: the uPA/SCID mice and Fab(-/-) Rag2(-/-) II2rg(-/-) mice. The lack of hardy breeding and the overly elaborated technique in these two models may hinder the potential future application of these models to hepatitis virus infection and vaccination studies. Improving the transplantation protocol for liver xeno-repopulation from human hepatocytes will increase the model efficiency and application. In this study, we successfully apply immunosuppressive drug treatments of anti-asialo GMI and FK506 in Fab(-/-) Rag2(-/-) mice, resulting in significant liver xeno-repopulation from human hepatocytes and human fetal liver cells. This methodology decreases the risk of animal mortality during breeding and surgery. When infected with hepatitis B virus (HBV) sera, Fab(-/-) Rag2(-/-) mice with liver xeno-repopulation from human hepatocytes accumulate significant levels of HBV DNA and FIBV proteins. Our new protocol for humanized liver could be applied in the study of human hepatitis virus infection in vivo, as well as the pharma- cokinetics and efficacy of potential vaccines. (Am J Pathol 2010. 177:1311-1319; 10.2353/aypath.2010.091154) |
| 学科主题 | Pathology |
| 类目[WOS] | Pathology |
| 关键词[WOS] | TYROSINEMIA TYPE-I ; HEPATITIS-B-VIRUS ; FETAL LIVER ; RAT-LIVER ; STEM/PROGENITOR CELLS ; MOUSE-LIVER ; TRANSPLANTATION ; INFECTION ; ADULT ; VIVO |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000281717700027 |
| 版本 | 出版稿 |
| 源URL | [http://202.127.25.143/handle/331003/968]  |
| 专题 | 上海生化细胞研究所_上海生科院生化细胞研究所 |
| 推荐引用方式 GB/T 7714 | He, ZY,Zhang, HB,Zhang, X,et al. Liver Xeno-Repopulation with Human Hepatocytes in Fah(-/-) Rag2(-/-) Mice after Pharmacological Immunosuppression[J]. AMERICAN JOURNAL OF PATHOLOGY,2010,177(3):1311-1319. |
| APA | He, ZY.,Zhang, HB.,Zhang, X.,Xie, DF.,Chen, YX.,...&Wang, X.(2010).Liver Xeno-Repopulation with Human Hepatocytes in Fah(-/-) Rag2(-/-) Mice after Pharmacological Immunosuppression.AMERICAN JOURNAL OF PATHOLOGY,177(3),1311-1319. |
| MLA | He, ZY,et al."Liver Xeno-Repopulation with Human Hepatocytes in Fah(-/-) Rag2(-/-) Mice after Pharmacological Immunosuppression".AMERICAN JOURNAL OF PATHOLOGY 177.3(2010):1311-1319. |
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