Evidence that the amino acid residue Cys117 of chloroplastic monodehydroascorbate reductase is involved in its activity and structural stability
文献类型：期刊论文
| 作者 | Li, F; Wu, QY; Sun, YL; Ma, NN; Wang, XY; Meng, QW |
| 刊名 | INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
 |
| 出版日期 | 2010 |
| 卷号 | 46期号:3页码:350-355 |
| 关键词 | Monodehydroascorbate reductase Mutagenesis Structural stability Activity |
| 通讯作者 | Meng, QW (reprint author), Shandong Agr Univ, Coll Life Sci, State Key Lab Crop Biol, Tai An 271018, Shandong, Peoples R China., |
| 英文摘要 | Monodehydroascorbate reductase (MDAR, EC 1 6 5 4) is crucial for AsA regeneration and essential for maintaining the reduced pool of AsA And the amino acid residue C117 of chloroplastic MDAR is the conserved cysteine residue in MDAR isoforms A series mutation of conserved amino acid residue cysteine 117 (C117) was constructed to investigate its role in MDAR structural stability and activity Our study revealed that mutation in this conserved residue could cause pronounced loss of activity and conformational changes Spectroscopic experiments indicated that these mutations influenced transition from the molten globule intermediate to the native state in folding process These results suggested that amino acid residue C117 played a relatively important role in keeping MDAR structural stability and activity (C) 2010 Elsevier B.V. All rights reserved |
| 学科主题 | Biochemistry & Molecular Biology |
| 类目[WOS] | Biochemistry & Molecular Biology |
| 关键词[WOS] | FREE-RADICAL REDUCTASE ; CRYSTAL-STRUCTURE ; MOLECULAR-CLONING ; CDNA CLONING ; ROOT-NODULES ; ASCORBATE ; PROTEIN ; PURIFICATION ; SPINACH ; ENZYMES |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000276381300012 |
| 版本 | 出版稿 |
| 源URL | [http://202.127.25.143/handle/331003/973]  |
| 专题 | 上海生化细胞研究所_上海生科院生化细胞研究所 |
| 推荐引用方式 GB/T 7714 | Li, F,Wu, QY,Sun, YL,et al. Evidence that the amino acid residue Cys117 of chloroplastic monodehydroascorbate reductase is involved in its activity and structural stability[J]. INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES,2010,46(3):350-355. |
| APA | Li, F,Wu, QY,Sun, YL,Ma, NN,Wang, XY,&Meng, QW.(2010).Evidence that the amino acid residue Cys117 of chloroplastic monodehydroascorbate reductase is involved in its activity and structural stability.INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES,46(3),350-355. |
| MLA | Li, F,et al."Evidence that the amino acid residue Cys117 of chloroplastic monodehydroascorbate reductase is involved in its activity and structural stability".INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES 46.3(2010):350-355. |
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