Essential Role of a Trypanosome U4-Specific Sm Core Protein in Small Nuclear Ribonucleoprotein Assembly and Splicing
文献类型：期刊论文
| 作者 | Jae, N; Wang, PP; Gu, TP; Huhn, M; Palfi, Z; Urlaub, H; Bindereif, A |
| 刊名 | EUKARYOTIC CELL
 |
| 出版日期 | 2010 |
| 卷号 | 9期号:3页码:379-386 |
| 通讯作者 | Bindereif, A (reprint author), Univ Giessen, Inst Biochem, Heinrich Buff Ring 58, D-35392 Giessen, Germany.,albrecht.bindereif@chemie.bio.uni-giessen.de |
| 英文摘要 | Spliceosomal small nuclear ribonucleoproteins (snRNPs) in trypanosomes contain either the canonical heptameric Sm ring or variant Sm cores with snRNA-specific Sm subunits. Here we show biochemically by a combination of RNase H cleavage and tandem affinity purification that the U4 snRNP contains a variant Sm heteroheptamer core in which only SmD3 is replaced by SSm4. This U4-specific, nuclear-localized Sm core protein is essential for growth and splicing. As shown by RNA interference (RNAi) knockdown, SSm4 is specifically required for the integrity of the U4 snRNA and the U4/U6 di-snRNP in trypanosomes. In addition, we demonstrate by in vitro reconstitution of Sm cores that under stringent conditions, the SSm4 protein suffices to specify the assembly of U4 Sm cores. Together, these data indicate that the assembly of the U4-specific Sm core provides an essential step in U4/U6 di-snRNP biogenesis and splicing in trypanosomes. |
| 学科主题 | Microbiology; Mycology |
| 类目[WOS] | Microbiology ; Mycology |
| 关键词[WOS] | U6 SNRNA ; CYTOPLASMIC FOCI ; LEADER RNA ; U7 SNRNPS ; IN-VITRO ; BRUCEI ; IDENTIFICATION ; COMPLEXES ; GENE ; TRANS |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000275108200003 |
| 版本 | 出版稿 |
| 源URL | [http://202.127.25.143/handle/331003/977]  |
| 专题 | 上海生化细胞研究所_上海生科院生化细胞研究所 |
| 推荐引用方式 GB/T 7714 | Jae, N,Wang, PP,Gu, TP,et al. Essential Role of a Trypanosome U4-Specific Sm Core Protein in Small Nuclear Ribonucleoprotein Assembly and Splicing[J]. EUKARYOTIC CELL,2010,9(3):379-386. |
| APA | Jae, N.,Wang, PP.,Gu, TP.,Huhn, M.,Palfi, Z.,...&Bindereif, A.(2010).Essential Role of a Trypanosome U4-Specific Sm Core Protein in Small Nuclear Ribonucleoprotein Assembly and Splicing.EUKARYOTIC CELL,9(3),379-386. |
| MLA | Jae, N,et al."Essential Role of a Trypanosome U4-Specific Sm Core Protein in Small Nuclear Ribonucleoprotein Assembly and Splicing".EUKARYOTIC CELL 9.3(2010):379-386. |
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