3T3-L1 adipocyte apoptosis induced by thiazolidinediones is peroxisome proliferator-activated receptor-gamma-dependent and mediated by the caspase-3-dependent apoptotic pathway
文献类型：期刊论文
| 作者 | Xiao, YY; Yuan, TC; Yao, WQ; Liao, K |
| 刊名 | FEBS JOURNAL
 |
| 出版日期 | 2010 |
| 卷号 | 277期号:3页码:687-696 |
| 关键词 | 3T3-L1 adipocyte adipocyte apoptosis Akt-1 PPAR gamma thiazolidinediones |
| 通讯作者 | Liao, K (reprint author), Chinese Acad Sci, Shanghai Inst Biol Sci, Inst Biochem & Cell Biol, State Key Lab Mol Biol, 320 Yueyang Rd, Shanghai 200031, Peoples R China.,kliao@sibs.ac.cn |
| 英文摘要 | Although thiazolidinediones (TZDs) are potent promoters of adipogenesis in the preadipocyte, they induce apoptosis in several other cell types, such as cancer cells, endothelial cells and T-lymphocytes. In this study, we investigated the proapoptotic effect of TZDs in mature 3T3-L1 adipocytes, which express high levels of the peroxisome proliferator-activated receptor-gamma (PPAR gamma) protein. Apoptosis was induced in mature 3T3-L1 adipocytes by treatment with troglitazone, pioglitazone or prostaglandin J2, and could be blocked by the PPAR gamma antagonist GW9662. Treatment with PPAR gamma agonists also decreased Akt-1 protein and phosphorylation levels without affecting phosphoinositide 3-kinase and PTEN. Further analysis indicated that in troglitazone-treated 3T3-L1 adipocytes, Bad phosphorylation and Bcl-2 protein levels were reduced, and Bax translocation to the mitochondria was increased. Subsequently, cytochrome c release and caspase-3 cleavage were observed. TZD-induced adipocyte apoptosis could be blocked by the caspase-3 inhibitor Ac-DEVD-CHO or by overexpression of Bcl2. In cultured rat primary adipocytes, similar apoptosis-inducing effects of troglitazone were also observed. Thus, TZDs promote apoptosis in adipocytes through a PPAR gamma-dependent pathway. This apoptosis is mediated by the inhibition of Akt-1, which decreases Bad phosphorylation and activates the mitochondrial apoptotic pathway. |
| 学科主题 | Biochemistry & Molecular Biology |
| 类目[WOS] | Biochemistry & Molecular Biology |
| 关键词[WOS] | ADIPOSE CELL LINE ; PPAR-GAMMA ; PROTEIN-KINASE ; DIFFERENTIATION ; ADIPOGENESIS ; SURVIVAL ; GROWTH ; AGONISTS ; CULTURE ; CANCER |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000273723200016 |
| 版本 | 出版稿 |
| 源URL | [http://202.127.25.143/handle/331003/983]  |
| 专题 | 上海生化细胞研究所_上海生科院生化细胞研究所 |
| 推荐引用方式 GB/T 7714 | Xiao, YY,Yuan, TC,Yao, WQ,et al. 3T3-L1 adipocyte apoptosis induced by thiazolidinediones is peroxisome proliferator-activated receptor-gamma-dependent and mediated by the caspase-3-dependent apoptotic pathway[J]. FEBS JOURNAL,2010,277(3):687-696. |
| APA | Xiao, YY,Yuan, TC,Yao, WQ,&Liao, K.(2010).3T3-L1 adipocyte apoptosis induced by thiazolidinediones is peroxisome proliferator-activated receptor-gamma-dependent and mediated by the caspase-3-dependent apoptotic pathway.FEBS JOURNAL,277(3),687-696. |
| MLA | Xiao, YY,et al."3T3-L1 adipocyte apoptosis induced by thiazolidinediones is peroxisome proliferator-activated receptor-gamma-dependent and mediated by the caspase-3-dependent apoptotic pathway".FEBS JOURNAL 277.3(2010):687-696. |
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