C-terminal amino acids 290-328 of LPTS/PinX1 confer telomerase inhibition
文献类型：期刊论文
| 作者 | Chen, GY; Da, LA; Xu, Y; Xu, MF; Song, L; Li, TP; Zhao, MJ |
| 刊名 | BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
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| 出版日期 | 2010 |
| 卷号 | 398期号:4页码:683-689 |
| 关键词 | LPTS/PinX1 Pin2/TRF1 Telomere length Telomerase Cell death |
| 通讯作者 | Zhao, MJ (reprint author), Chinese Acad Sci, Shanghai Inst Biol Sci, Inst Biochem & Cell Biol, State Key Lab Mol Biol, 320 Yueyang Rd, Shanghai 200031, Peoples R China.,mjzhao@sibs.ac.cn |
| 英文摘要 | LPTS/PinX1, a telomerase inhibitor composed of 328 amino acids, binds to the telomere associated protein Pin2/TRF1 and to the telomerase catalytic subunit hTERT. However, the mechanism by which LPTS/PinX1 regulates telomerase activity remains unclear. Here we show, for the first time, that LPTS/PinX1 uses different domains to interact with Pin2/TRF1 and hTERT. The LPTS/PinX1(254-289) fragment specifically binds to Pin2/TRF1, and LPTS/PinX1(290-328) can associate with hTERT. Compared with the full-length LPTS/PinX1 protein, LPTS/PinX1(290-328) shows stronger in vitro telomerase inhibitory activity. Moreover, the LPTS/PinX1 protein was recruited to telomeres for binding to Pin2/TRF1. Overexpression of LPTS/PinX1(290-328), which contains a nucleolus localization signal, in cells resulted in telomere shortening and progressive cell death. Conversely, telomere elongation was induced by expression of the dominant-negative LPTS/PinX1(1-289). Our results suggest that the C-terminal fragment of LPTS/PinX1 (LPTS/PinX1(290-328)) contains a telomerase inhibitory domain that is required for the inhibition of telomere elongation and the induction of cell crisis. Our studies also provide evidence that LPTS/PinX1 interaction with Pin2/TRF1 may play a role in the stabilization of telomeres. (c) 2010 Elsevier Inc. All rights reserved. |
| 学科主题 | Biochemistry & Molecular Biology ; Biophysics |
| 类目[WOS] | Biochemistry & Molecular Biology ; Biophysics |
| 关键词[WOS] | PINX1 ; TRF1 ; RNA |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000280901300012 |
| 版本 | 出版稿 |
| 源URL | [http://202.127.25.143/handle/331003/988]  |
| 专题 | 上海生化细胞研究所_上海生科院生化细胞研究所 |
| 推荐引用方式 GB/T 7714 | Chen, GY,Da, LA,Xu, Y,et al. C-terminal amino acids 290-328 of LPTS/PinX1 confer telomerase inhibition[J]. BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS,2010,398(4):683-689. |
| APA | Chen, GY.,Da, LA.,Xu, Y.,Xu, MF.,Song, L.,...&Zhao, MJ.(2010).C-terminal amino acids 290-328 of LPTS/PinX1 confer telomerase inhibition.BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS,398(4),683-689. |
| MLA | Chen, GY,et al."C-terminal amino acids 290-328 of LPTS/PinX1 confer telomerase inhibition".BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS 398.4(2010):683-689. |
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