ALL-TRANS RETINOIC ACID AFFECTS SUBCELLULAR LOCALIZATION OF A NOVEL BmNIF3l PROTEIN: FUNCTIONAL DEDUCE AND TISSUE DISTRIBUTION OF NIF3l GENE FROM SILKWORM (Bombyx mori)
文献类型：期刊论文
| 作者 | Chen, JQ; Gai, QJ; Lv, ZB; Chen, JA; Nie, ZM; Wu, XF; Zhang, YZ |
| 刊名 | ARCHIVES OF INSECT BIOCHEMISTRY AND PHYSIOLOGY
 |
| 出版日期 | 2010 |
| 卷号 | 74期号:4页码:217-231 |
| 关键词 | Bombyx mori NIF3l expression pattern all-trans retinoic acid subcellular localization |
| 通讯作者 | Zhang, YZ (reprint author), Zhejiang Sci Tech Univ, Inst Biochem, 2nd St,Xiasha Higher Educ Zone, Hangzhou 310018, Zhejiang, Peoples R China.,yaozhou@zstu.edu.cn |
| 英文摘要 | A novel cDNA sequence encoding a predicted protein of 271 amino acids containing a conserved NIF3 domain was found from a pupal cDNA library of silkworm. The corresponding gene was named BmNIF3l (Bombyx mori NGG1p interacting factor 3-like). It was found by bioinformatics that BmNIF3l gene consisted of five exons and four introns and BmNIF3l had a high degree of homology to other NIF3-like proteins, especially in the N-terminal and C-terminal regions. A His-tagged BmNIF3l fusion protein with a molecular weight of approximately 33.6 kDa was expressed and purified to homogeneity. We have used the purified fusion protein to produce polyclonal antibodies against BmNIF3l for histochemical analysis. Subcellular localization revealed that BmNIF3l is a cytoplasmic protein that responds to all-trans retinoic acid (ATRA). Western blotting and real-time reverse transcription polymerase chain reaction showed that the expression level of BmNIF3l is higher in tissues undergoing differentiation. Taken together, the results suggest that BmNIF3l functions in transcription. (C) 2010 Wiley Periodicals, Inc. |
| 学科主题 | Biochemistry & Molecular Biology; Entomology; Physiology |
| 类目[WOS] | Biochemistry & Molecular Biology ; Entomology ; Physiology |
| 关键词[WOS] | SACCHAROMYCES-CEREVISIAE ; BINDING PROTEIN ; CRITICAL REGION ; EXPRESSION |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000281399500002 |
| 版本 | 出版稿 |
| 源URL | [http://202.127.25.143/handle/331003/989]  |
| 专题 | 上海生化细胞研究所_上海生科院生化细胞研究所 |
| 推荐引用方式 GB/T 7714 | Chen, JQ,Gai, QJ,Lv, ZB,et al. ALL-TRANS RETINOIC ACID AFFECTS SUBCELLULAR LOCALIZATION OF A NOVEL BmNIF3l PROTEIN: FUNCTIONAL DEDUCE AND TISSUE DISTRIBUTION OF NIF3l GENE FROM SILKWORM (Bombyx mori)[J]. ARCHIVES OF INSECT BIOCHEMISTRY AND PHYSIOLOGY,2010,74(4):217-231. |
| APA | Chen, JQ.,Gai, QJ.,Lv, ZB.,Chen, JA.,Nie, ZM.,...&Zhang, YZ.(2010).ALL-TRANS RETINOIC ACID AFFECTS SUBCELLULAR LOCALIZATION OF A NOVEL BmNIF3l PROTEIN: FUNCTIONAL DEDUCE AND TISSUE DISTRIBUTION OF NIF3l GENE FROM SILKWORM (Bombyx mori).ARCHIVES OF INSECT BIOCHEMISTRY AND PHYSIOLOGY,74(4),217-231. |
| MLA | Chen, JQ,et al."ALL-TRANS RETINOIC ACID AFFECTS SUBCELLULAR LOCALIZATION OF A NOVEL BmNIF3l PROTEIN: FUNCTIONAL DEDUCE AND TISSUE DISTRIBUTION OF NIF3l GENE FROM SILKWORM (Bombyx mori)".ARCHIVES OF INSECT BIOCHEMISTRY AND PHYSIOLOGY 74.4(2010):217-231. |
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