Development of a low-cost detection method for miRNA microarray
文献类型：期刊论文
| 作者 | Li, W; Zhao, BT; Jin, YX; Ruan, KC |
| 刊名 | ACTA BIOCHIMICA ET BIOPHYSICA SINICA
 |
| 出版日期 | 2010 |
| 卷号 | 42期号:4页码:296-301 |
| 关键词 | microRNA miRNA microarray enzyme-linked detection method of miRNA |
| 通讯作者 | Ruan, KC (reprint author), Chinese Acad Sci, Shanghai Inst Biol Sci, Inst Biochem & Cell Biol, State Key Lab Mol Biol, Shanghai 200031, Peoples R China.,kcruan@sibs.ac.cn |
| 英文摘要 | MicroRNA (miRNA) microarray is a powerful tool to explore the expression profiling of miRNA. The current detection method used in miRNA microarray is mainly fluorescence based, which usually requires costly detection system such as laser confocal scanner of tens of thousands of dollars. Recently, we developed a low-cost yet sensitive detection method for miRNA microarray based on enzyme-linked assay. In this approach, the biotinylated miRNAs were captured by the corresponding oligonucleotide probes immobilized on microarray slide; and then the biotinylated miRNAs would capture streptavidin-conjugated alkaline phosphatase. A purple-black precipitation on each biotinylated miRNA spot was produced by the enzyme catalytic reaction. It could be easily detected by a charge-coupled device digital camera mounted on a microscope, which lowers the detection cost more than 100 fold compared with that of fluorescence method. Our data showed that signal intensity of the spot correlates well with the biotinylated miRNA concentration and the detection limit for miRNAs is at least 0.4 fmol and the detection dynamic range spans about 2.5 orders of magnitude, which is comparable to that of fluorescence method. |
| 学科主题 | Biochemistry & Molecular Biology; Biophysics |
| 类目[WOS] | Biochemistry & Molecular Biology ; Biophysics |
| 关键词[WOS] | MICRORNA GENE-EXPRESSION ; OLIGONUCLEOTIDE MICROARRAY ; CAENORHABDITIS-ELEGANS ; NANOGOLD PROBE ; SIGNATURES ; PLATFORM ; REVEALS ; ENCODES ; TISSUES |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000276287400009 |
| 版本 | 出版稿 |
| 源URL | [http://202.127.25.143/handle/331003/991]  |
| 专题 | 上海生化细胞研究所_上海生科院生化细胞研究所 |
| 推荐引用方式 GB/T 7714 | Li, W,Zhao, BT,Jin, YX,et al. Development of a low-cost detection method for miRNA microarray[J]. ACTA BIOCHIMICA ET BIOPHYSICA SINICA,2010,42(4):296-301. |
| APA | Li, W,Zhao, BT,Jin, YX,&Ruan, KC.(2010).Development of a low-cost detection method for miRNA microarray.ACTA BIOCHIMICA ET BIOPHYSICA SINICA,42(4),296-301. |
| MLA | Li, W,et al."Development of a low-cost detection method for miRNA microarray".ACTA BIOCHIMICA ET BIOPHYSICA SINICA 42.4(2010):296-301. |
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