Overexpression of chloroplastic monodehydroascorbate reductase enhanced tolerance to temperature and methyl viologen-mediated oxidative stresses
文献类型：期刊论文
| 作者 | Li, F; Wu, QY; Sun, YL; Wang, LY; Yang, XH; Meng, QW |
| 刊名 | PHYSIOLOGIA PLANTARUM
 |
| 出版日期 | 2010 |
| 卷号 | 139期号:4页码:421-434 |
| 通讯作者 | Meng, QW (reprint author), Shandong Agr Univ, State Key Lab Crop Biol, Coll Life Sci, Tai An 271018, Shandong, Peoples R China.,qwmeng@sdau.edu.cn |
| 英文摘要 | A tomato (Lycopersicon esculentum Mill.) monodehydroascorbate reductase gene (LeMDAR) was isolated. The LeMDAR-green fluorescence protein (GFP) fusion protein was targeted to chloroplast in Arabidopsis mesophyll protoplast. RNA and protein gel blot analyses confirmed that the sense- and antisense- LeMDAR were integrated into the tomato genome. The MDAR activities and the levels of reduced ascorbate (AsA) were markedly increased in sense transgenic lines and decreased in antisense transgenic lines compared with wild-type (WT) plants. Under low and high temperature stresses, the sense transgenic plants showed lower level of hydrogen peroxide (H(2)O(2)), lower thiobarbituric acid reactive substance (TBARS) content, higher net photosynthetic rate (P(n)), higher maximal photochemical efficiency of PSII (F(v)/F(m)) and fresh weight compared with WT plants. The oxidizable P700 decreased more obviously in WT and antisense plants than that in sense plants at chilling temperature under low irradiance. Furthermore, the sense transgenic plants exhibited significantly lower H(2)O(2) level, higher ascorbate peroxidase (APX) activity, greater P(n) and F(v)/F(m) under methyl viologen (MV)-mediated oxidative stresses. These results indicated that overexpression of chloroplastic MDAR played an important role in alleviating photoinhibition of PSI and PSII and enhancing the tolerance to various abiotic stresses by elevating AsA level. |
| 学科主题 | Plant Sciences |
| 类目[WOS] | Plant Sciences |
| 关键词[WOS] | GLYCEROL-3-PHOSPHATE ACYLTRANSFERASE GENE ; DEFICIENT ARABIDOPSIS MUTANT ; DEHYDROASCORBATE REDUCTASE ; TOBACCO PLANTS ; ASCORBATE PEROXIDASE ; SPINACH-CHLOROPLASTS ; HYDROGEN-PEROXIDE ; CHILLING STRESS ; SUPEROXIDE-DISMUTASE ; ANTIOXIDANT ENZYMES |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000279840300008 |
| 版本 | 出版稿 |
| 源URL | [http://202.127.25.143/handle/331003/999]  |
| 专题 | 上海生化细胞研究所_上海生科院生化细胞研究所 |
| 推荐引用方式 GB/T 7714 | Li, F,Wu, QY,Sun, YL,et al. Overexpression of chloroplastic monodehydroascorbate reductase enhanced tolerance to temperature and methyl viologen-mediated oxidative stresses[J]. PHYSIOLOGIA PLANTARUM,2010,139(4):421-434. |
| APA | Li, F,Wu, QY,Sun, YL,Wang, LY,Yang, XH,&Meng, QW.(2010).Overexpression of chloroplastic monodehydroascorbate reductase enhanced tolerance to temperature and methyl viologen-mediated oxidative stresses.PHYSIOLOGIA PLANTARUM,139(4),421-434. |
| MLA | Li, F,et al."Overexpression of chloroplastic monodehydroascorbate reductase enhanced tolerance to temperature and methyl viologen-mediated oxidative stresses".PHYSIOLOGIA PLANTARUM 139.4(2010):421-434. |
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