Snapshot of the interaction between HIV envelope glycoprotein 120 and protein disulfide isomerase
文献类型：期刊论文
| 作者 | Wang, ZQ; Zhou, ZM; Guo, ZY; Chi, CW |
| 刊名 | ACTA BIOCHIMICA ET BIOPHYSICA SINICA
 |
| 出版日期 | 2010 |
| 卷号 | 42期号:5页码:358-362 |
| 关键词 | protein disulfide isomerase glycoprotein 120 kinetic trapping disulfide bond |
| 通讯作者 | Guo, ZY (reprint author), Tongji Univ, Inst Prot Res, Coll Life Sci & Technol, Shanghai 200092, Peoples R China.,zhan-yun.guo@tongji.edu.cn ; zwqi@sibs.ac.cn |
| 英文摘要 | The human immunodeficiency virus-1 (HIV-1) envelope glycoprotein 120 (gp120) binds to cell surface receptors and mediates HIV entry. Previous studies suggest the cell surface protein disulfide isomerase (PDI) might interact with disulfide bond(s) of gp120 and thus facilitate HIV-1 entry. In the present study, a kinetic trapping approach was used to capture the disulfide cross-linking intermediate between gp120 and PDI. Active site mutant PDIs were prepared in which the C-terminal cysteine at the active site was replaced by a serine. The active site mutant PDIs were able to covalently cross-link with gp120 through a mixed disulfide bond in vitro. The cross-linking efficiency was enhanced by CD4 protein (primary receptor of HIV-1) and was inhibited both by bacitracin (a PDI inhibitor) and by catalytically inactive PDI. The present results suggested the cell surface PDI might play a role in HIV entry in vivo. |
| 学科主题 | Biochemistry & Molecular Biology; Biophysics |
| 类目[WOS] | Biochemistry & Molecular Biology ; Biophysics |
| 关键词[WOS] | MEDIATED REDUCTION ; PDI FAMILY ; BONDS ; FUSION ; THIOREDOXIN ; INHIBITION ; EXCHANGE ; ENTRY ; GLUTATHIONE ; SWITCHES |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000278421900009 |
| 版本 | 出版稿 |
| 源URL | [http://202.127.25.143/handle/331003/1005]  |
| 专题 | 上海生化细胞研究所_上海生科院生化细胞研究所 |
| 推荐引用方式 GB/T 7714 | Wang, ZQ,Zhou, ZM,Guo, ZY,et al. Snapshot of the interaction between HIV envelope glycoprotein 120 and protein disulfide isomerase[J]. ACTA BIOCHIMICA ET BIOPHYSICA SINICA,2010,42(5):358-362. |
| APA | Wang, ZQ,Zhou, ZM,Guo, ZY,&Chi, CW.(2010).Snapshot of the interaction between HIV envelope glycoprotein 120 and protein disulfide isomerase.ACTA BIOCHIMICA ET BIOPHYSICA SINICA,42(5),358-362. |
| MLA | Wang, ZQ,et al."Snapshot of the interaction between HIV envelope glycoprotein 120 and protein disulfide isomerase".ACTA BIOCHIMICA ET BIOPHYSICA SINICA 42.5(2010):358-362. |
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