Label-free detection of oligonucleotide microarrays by oblique-incidence reflectivity difference method
文献类型：期刊论文
| 作者 | Wang, X; Yuan, K; Lu, H; Wen, J; Lu, HB; Jin, KJ; Zhou, YL; Yang, GZ; Li, W; Ruan, KC |
| 刊名 | JOURNAL OF APPLIED PHYSICS
 |
| 出版日期 | 2010 |
| 卷号 | 107期号:6页码:63109-63109 |
| 关键词 | biological techniques DNA fluorescence molecular biophysics |
| 通讯作者 | Yang, GZ (reprint author), Chinese Acad Sci, Inst Phys, Beijing Natl Lab Condensed Matter Phys, Beijing 100190, Peoples R China.,yanggz@aphy.iphy.ac.cn ; kcruan@sibs.ac.cn |
| 英文摘要 | Hybridizations between labeled or label-free targets and corresponding 21-base oligonucleotide probes, concentrations of which range from 0.39 to 50 mu M, are detected by oblique-incidence reflectivity difference (OI-RD) method and fluorescence detection. The experimental results demonstrate that the OI-RD method can be utilized to not only distinguish whether the hybridization of oligonucleotides happened but also directly tell the different concentrations of the labeled and unlabeled oligonucleotides on the microarrays. The analysis with a classical three-layer model suggests that single-strand DNA tends to lie on epoxy-functionalized glass slide while the double-strand DNA prefers to have a tilted angle with respect to the slide in our experimental situation. The label-free detection of hybridization of oligonucleotides declares that OI-RD is a promising method for label-free and high-throughput detection of the biological microarrays. |
| 学科主题 | Physics |
| 类目[WOS] | Physics, Applied |
| 关键词[WOS] | DNA ; PROBES ; FILMS |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000276210800017 |
| 版本 | 出版稿 |
| 源URL | [http://202.127.25.143/handle/331003/1010]  |
| 专题 | 上海生化细胞研究所_上海生科院生化细胞研究所 |
| 推荐引用方式 GB/T 7714 | Wang, X,Yuan, K,Lu, H,et al. Label-free detection of oligonucleotide microarrays by oblique-incidence reflectivity difference method[J]. JOURNAL OF APPLIED PHYSICS,2010,107(6):63109-63109. |
| APA | Wang, X.,Yuan, K.,Lu, H.,Wen, J.,Lu, HB.,...&Ruan, KC.(2010).Label-free detection of oligonucleotide microarrays by oblique-incidence reflectivity difference method.JOURNAL OF APPLIED PHYSICS,107(6),63109-63109. |
| MLA | Wang, X,et al."Label-free detection of oligonucleotide microarrays by oblique-incidence reflectivity difference method".JOURNAL OF APPLIED PHYSICS 107.6(2010):63109-63109. |
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