Lipopolysaccharide-Induced Epididymitis Disrupts Epididymal Beta-Defensin Expression and Inhibits Sperm Motility in Rats
文献类型：期刊论文
| 作者 | Cao, DM; Li, YD; Yang, R; Wang, Y; Zhou, YC; Diao, H; Zhao, Y; Zhang, YL; Lu, JA |
| 刊名 | BIOLOGY OF REPRODUCTION
 |
| 出版日期 | 2010 |
| 卷号 | 83期号:6页码:1064-1070 |
| 关键词 | beta-defensin epididymis gene regulation lipopolysaccharide sperm motility and transport |
| 通讯作者 | Lu, JA (reprint author), Second Mil Med Univ, Dept Pathophysiol, Shanghai, Peoples R China.,ylzhang@sibs.ac.cn ; lujian326@163.com |
| 英文摘要 | Although more than 40 beta-defensins have been identified in rat epididymis, little is known about their regulation or their relation to male infertility caused by inflammation. Using a rat model of epididymitis induced by lipopolysaccharide (LPS), we examined expression of SPAG11E (also known as Bin1b), a caput epididymis-specific beta-defensin in rat. Unlike the expression of other beta-defensins in various epithelial cells with upregulated expression after LPS stimulation, expression of SPAG11E was significantly decreased by LPS at the mRNA and protein levels. LPS treatment also significantly decreased both sperm binding to SPAG11E and sperm motility, and supplementation of the spermatozoa with recombinant SPAG11E in vitro remarkably increased both SPAG11E binding and motility of sperm. To clarify whether decreased expression is a common pattern of epididymal beta-defensins after LPS stimulation, we examined the expression of another 12 epididymal beta-defensins expressed in the caput epididymis. For nine of these beta-defensins, expression was decreased, but for the other three, expression remained unaffected. These findings demonstrate that LPS-induced epididymitis can decrease the expression of epididymal beta-defensins and that disruption of SPAG11E expression is involved in the impairment of sperm motility. |
| 学科主题 | Reproductive Biology |
| 类目[WOS] | Reproductive Biology |
| 关键词[WOS] | ESCHERICHIA-COLI EPIDIDYMITIS ; INTESTINAL EPITHELIAL-CELLS ; EXCURRENT DUCTAL SYSTEM ; GENE-EXPRESSION ; REPRODUCTIVE-TRACT ; INFLAMMATORY CONDITIONS ; MESSENGER-RNA ; FAMILY ; PROTEINS ; MICE |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000284737600022 |
| 版本 | 出版稿 |
| 源URL | [http://202.127.25.143/handle/331003/1011]  |
| 专题 | 上海生化细胞研究所_上海生科院生化细胞研究所 |
| 推荐引用方式 GB/T 7714 | Cao, DM,Li, YD,Yang, R,et al. Lipopolysaccharide-Induced Epididymitis Disrupts Epididymal Beta-Defensin Expression and Inhibits Sperm Motility in Rats[J]. BIOLOGY OF REPRODUCTION,2010,83(6):1064-1070. |
| APA | Cao, DM.,Li, YD.,Yang, R.,Wang, Y.,Zhou, YC.,...&Lu, JA.(2010).Lipopolysaccharide-Induced Epididymitis Disrupts Epididymal Beta-Defensin Expression and Inhibits Sperm Motility in Rats.BIOLOGY OF REPRODUCTION,83(6),1064-1070. |
| MLA | Cao, DM,et al."Lipopolysaccharide-Induced Epididymitis Disrupts Epididymal Beta-Defensin Expression and Inhibits Sperm Motility in Rats".BIOLOGY OF REPRODUCTION 83.6(2010):1064-1070. |
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