A whole-mechanical method to establish human embryonic stem cell line HN4 from discarded embryos
文献类型：期刊论文
| 作者 | Li, B; Xu, L; Lu, WY; Xu, W; Wang, MH; Yang, K; Dong, JA; Ding, XY; Huang, YH |
| 刊名 | CYTOTECHNOLOGY
 |
| 出版日期 | 2010 |
| 卷号 | 62期号:6页码:509-518 |
| 关键词 | Human stem cells ICM mechanical isolation Mixed feeder layer Neural differentiation |
| 通讯作者 | Huang, YH (reprint author), Hainan Med Coll, Reprod Med Ctr Hainan Prov, Haikou 570102, Peoples R China.,libinliccc@163.com ; xl_any@163.com ; xyding@sunm.shcnc.ac.cn ; huangyh4180@sohu.com |
| 英文摘要 | Since the first human embryonic stem cell (hESC) line was generated by Thomson et al. (in Science 282:1145-1147, 1998), hundreds of hESC lines have been reported by different labs, providing resources for basic research and regenerative medicine as well. However it has been widely recognized that hESC lines varied on their properties, in terms of gene expression profile, epigenetic modify profile, and differentiation tendency. Generation of more hESC lines will largely enhance our knowledge of hESCs innate character. In this current work, we reported the generation of HN4, a hESC line derived from grade III IVF human embryo by using a mixture of human foreskin fibroblast (HFF) and mouse embryonic fibroblast (MEF) as feeder layers, and a whole-mechanical method in inner cell mass (ICM) isolation. HN4 satisfied the criteria of hESCs pluripotency, with high expression of hESC surface markers (SSEA-3, SSEA-4, TRA-1-60, TRA-1-81), transcription factors (OCT-4, NANOG, REX-1), and alkaline phosphatase. It is able to differentiate to three germ layer derivatives when cultured in vitro, or in teratoma formation. Moreover, it displayed promising potential in neural differentiation under a proper culture condition, suggesting the advantage of HN4 in further investigation. Additionally, the whole-mechanical protocol for ICM isolation facilitates hESC line generation for its ease to handle. |
| 学科主题 | Biotechnology & Applied Microbiology; Cell Biology |
| 类目[WOS] | Biotechnology & Applied Microbiology ; Cell Biology |
| 关键词[WOS] | PROLONGED UNDIFFERENTIATED GROWTH ; IN-VITRO DIFFERENTIATION ; LONG-TERM PROLIFERATION ; NEURAL PRECURSORS ; HUMAN BLASTOCYSTS ; HUMAN FEEDERS ; DERIVATION ; CULTURE ; MAINTENANCE ; THERAPIES |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000284844300004 |
| 版本 | 出版稿 |
| 源URL | [http://202.127.25.143/handle/331003/1012]  |
| 专题 | 上海生化细胞研究所_上海生科院生化细胞研究所 |
| 推荐引用方式 GB/T 7714 | Li, B,Xu, L,Lu, WY,et al. A whole-mechanical method to establish human embryonic stem cell line HN4 from discarded embryos[J]. CYTOTECHNOLOGY,2010,62(6):509-518. |
| APA | Li, B.,Xu, L.,Lu, WY.,Xu, W.,Wang, MH.,...&Huang, YH.(2010).A whole-mechanical method to establish human embryonic stem cell line HN4 from discarded embryos.CYTOTECHNOLOGY,62(6),509-518. |
| MLA | Li, B,et al."A whole-mechanical method to establish human embryonic stem cell line HN4 from discarded embryos".CYTOTECHNOLOGY 62.6(2010):509-518. |
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