Structural Analysis of Rtt106p Reveals a DNA Binding Role Required for Heterochromatin Silencing
文献类型：期刊论文
| 作者 | Liu, YW; Huang, HD; Zhou, BO; Wang, SS; Hu, YX; Li, X; Liu, JP; Zang, JY; Niu, LW; Wu, JH |
| 刊名 | JOURNAL OF BIOLOGICAL CHEMISTRY
 |
| 出版日期 | 2010 |
| 卷号 | 285期号:6页码:4251-4262 |
| 通讯作者 | Teng, MK (reprint author), Univ Sci & Technol China, Sch Life Sci, Hefei 230026, Anhui, Peoples R China.,mkteng@ustc.edu.cn ; yyshi@ustc.edu.cn |
| 英文摘要 | Rtt106p is a Saccharomyces cerevisiae histone chaperone with roles in heterochromatin silencing and nucleosome assembly. The molecular mechanism by which Rtt106p engages in chromatin dynamics remains unclear. Here, we report the 2.5 angstrom crystal structure of the core domain of Rtt106p, which adopts an unusual "double pleckstrin homology" domain architecture that represents a novel structural mode for histone chaperones. A histone H3-H4-binding region and a novel double-stranded DNA-binding region have been identified. Mutagenesis studies reveal that the histone and DNA binding activities of Rtt106p are involved in Sir protein-mediated heterochromatin formation. Our results uncover the structural basis of the diverse functions of Rtt106p and provide new insights into its cellular roles. |
| 学科主题 | Biochemistry & Molecular Biology |
| 类目[WOS] | Biochemistry & Molecular Biology |
| 关键词[WOS] | HISTONE CHAPERONE ACTIVITY ; ASSEMBLY FACTOR-I ; SACCHAROMYCES-CEREVISIAE ; TRANSCRIPTION ELONGATION ; MAMMALIAN-CELLS ; CHROMATIN ; PROTEIN ; YEAST ; FACT ; INHERITANCE |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000275254000077 |
| 版本 | 出版稿 |
| 源URL | [http://202.127.25.143/handle/331003/1024]  |
| 专题 | 上海生化细胞研究所_上海生科院生化细胞研究所 |
| 推荐引用方式 GB/T 7714 | Liu, YW,Huang, HD,Zhou, BO,et al. Structural Analysis of Rtt106p Reveals a DNA Binding Role Required for Heterochromatin Silencing[J]. JOURNAL OF BIOLOGICAL CHEMISTRY,2010,285(6):4251-4262. |
| APA | Liu, YW.,Huang, HD.,Zhou, BO.,Wang, SS.,Hu, YX.,...&Shi, YY.(2010).Structural Analysis of Rtt106p Reveals a DNA Binding Role Required for Heterochromatin Silencing.JOURNAL OF BIOLOGICAL CHEMISTRY,285(6),4251-4262. |
| MLA | Liu, YW,et al."Structural Analysis of Rtt106p Reveals a DNA Binding Role Required for Heterochromatin Silencing".JOURNAL OF BIOLOGICAL CHEMISTRY 285.6(2010):4251-4262. |
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