中国科学院机构知识库网格
Chinese Academy of Sciences Institutional Repositories Grid
PHF8 is a histone H3K9me2 demethylase regulating rRNA synthesis

文献类型:期刊论文

作者Zhu, ZQ; Wang, YR; Li, X; Wang, YQ; Xu, LY; Wang, X; Sun, TL; Dong, XB; Chen, LL; Mao, HL
刊名CELL RESEARCH
出版日期2010
卷号20期号:7页码:794-801
关键词PHF8 histone demethylase H3K9me2 rRNA synthesis
通讯作者Chen, PA (reprint author), Chinese Acad Sci, Shanghai Inst Biol Sci, Inst Biochem & Cell Biol, Shanghai Key Lab Mol Androl, 310 Yueyang Rd, Shanghai 100031, Peoples R China.,pinchen@sibs.ac.cn ; cdchen@sibs.ac.cn
英文摘要Dimethylation of histone H3 lysine 9 (H3K9me2) is an important epigenetic mark associated with transcription repression. Here, we identified PHF8, a JmjC-domain-containing protein, as a histone demethylase specific for this repressing mark. Recombinant full-length wild type protein could remove methylation from H3K9me2, but mutation of a conserved histidine to alanine H247A abolished the demethylase activity. Overexpressed exogenous PHF8 was colocalized with B23 staining. Endogenous PHF8 was also colocalized with B23 and fibrillarin, two well-established nucleolus proteins, suggesting that PHF8 is localized in the nucleolus and may regulate rRNA transcription. Indeed, PHF8 bound to the promoter region of the rDNA gene. Knockdown of PHF8 reduced the expression of rRNA, and overexpression of the gene resulted in upregulation of rRNA transcript. Concomitantly, H3K9me2 level was elevated in the promoter region of the rDNA gene in PHF8 knockdown cells and reduced significantly when the wild type but not the catalytically inactive H247A mutant PHF8 was overexpressed. Thus, our study identified a histone demethylase for H3K9me2 that regulates rRNA transcription.
学科主题Cell Biology
类目[WOS]Cell Biology
关键词[WOS]DOMAIN-CONTAINING PROTEINS ; STRUCTURAL INSIGHTS ; LYSINE DEMETHYLASE ; METHYLATION ; METHYLTRANSFERASE ; CHROMATIN ; GENES ; G9A ; TRANSCRIPTION ; DIMETHYL
收录类别SCI
语种英语
WOS记录号WOS:000280079500008
版本出版稿
源URL[http://202.127.25.143/handle/331003/1029]  
专题上海生化细胞研究所_上海生科院生化细胞研究所
推荐引用方式
GB/T 7714
Zhu, ZQ,Wang, YR,Li, X,et al. PHF8 is a histone H3K9me2 demethylase regulating rRNA synthesis[J]. CELL RESEARCH,2010,20(7):794-801.
APA Zhu, ZQ.,Wang, YR.,Li, X.,Wang, YQ.,Xu, LY.,...&Chen, CD.(2010).PHF8 is a histone H3K9me2 demethylase regulating rRNA synthesis.CELL RESEARCH,20(7),794-801.
MLA Zhu, ZQ,et al."PHF8 is a histone H3K9me2 demethylase regulating rRNA synthesis".CELL RESEARCH 20.7(2010):794-801.

入库方式: OAI收割

来源:上海生物化学与细胞生物学研究所

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