In Vitro Unfolding of Insulin: Characterization of Intermediates and Putative Unfolding Pathway
文献类型：期刊论文
| 作者 | Zhao, J; Huang, QL; Tang, YH; Zhao, FK; Feng, YM |
| 刊名 | PROTEIN AND PEPTIDE LETTERS
 |
| 出版日期 | 2010 |
| 卷号 | 17期号:7页码:874-880 |
| 关键词 | Disulfide folding insulin unfolding unfolding intermediate unfolding pathway |
| 通讯作者 | Feng, YM (reprint author), Zhejiang Sci Tech Univ, Coll Life Sci, Hangzhou 310018, Peoples R China.,fengym@sunm.shcnc.ac.cn |
| 英文摘要 | The in vitro insulin unfolding had been studied using the "equilibrium unfolding" method where protein is unfolded by reducing reagents in the presence of trace amounts of oxidants such as oxidized glutathione. Nine intermediates were captured in the unfolding process, named as P1A, P2A, P3A, P4A, P3B, P4B, P5B, P6B, and P7B, which were all either A chain derivatives or B chain derivatives. No intermediate with inter-A-B chain disulfide was captured. Based on the character of the intermediates, their distribution during the unfolding process and the hypothetic "transient" intermediates, an in vitro putative unfolding pathway of insulin had been proposed. Besides, the comparison of the intermediates captured in unfolding with the intermediates captured in the refolding process of insulin revealed that both unfolding/refolding processes of insulin shared common intermediates. Based on these observations we suggested that the unfolding pathway of insulin was similar to the refolding pathway but flowed in the opposite direction. |
| 学科主题 | Biochemistry & Molecular Biology |
| 类目[WOS] | Biochemistry & Molecular Biology |
| 关键词[WOS] | DISULFIDE-FORMING PATHWAY ; CARBOXYPEPTIDASE INHIBITOR ; HUMAN PROINSULIN ; RIBONUCLEASE-A ; C-PEPTIDE ; PRECURSOR ; PROTEINS ; MECHANISM |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000277910800010 |
| 版本 | 出版稿 |
| 源URL | [http://202.127.25.143/handle/331003/1032]  |
| 专题 | 上海生化细胞研究所_上海生科院生化细胞研究所 |
| 推荐引用方式 GB/T 7714 | Zhao, J,Huang, QL,Tang, YH,et al. In Vitro Unfolding of Insulin: Characterization of Intermediates and Putative Unfolding Pathway[J]. PROTEIN AND PEPTIDE LETTERS,2010,17(7):874-880. |
| APA | Zhao, J,Huang, QL,Tang, YH,Zhao, FK,&Feng, YM.(2010).In Vitro Unfolding of Insulin: Characterization of Intermediates and Putative Unfolding Pathway.PROTEIN AND PEPTIDE LETTERS,17(7),874-880. |
| MLA | Zhao, J,et al."In Vitro Unfolding of Insulin: Characterization of Intermediates and Putative Unfolding Pathway".PROTEIN AND PEPTIDE LETTERS 17.7(2010):874-880. |
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