E-Cadherin-Mediated Cell-Cell Contact Is Critical for Induced Pluripotent Stem Cell Generation
文献类型：期刊论文
| 作者 | Chen, TT; Yuan, DT; Wei, B; Jiang, J; Kang, JH; Ling, K; Gu, YJ; Li, JS; Xiao, L; Pei, G |
| 刊名 | STEM CELLS
 |
| 出版日期 | 2010 |
| 卷号 | 28期号:8页码:1315-1325 |
| 关键词 | Cell adhesion molecules Reprograming Induced pluripotent stem Embryonic stem cells |
| 通讯作者 | Pei, G (reprint author), Chinese Acad Sci, Mol Cell Biol Lab, Inst Biochem & Cell Biol, Shanghai Inst Biol Sci, Shanghai, Peoples R China.,gpei@sibs.ac.cn |
| 英文摘要 | The low efficiency of reprogramming and genomic integration of virus vectors obscure the potential application of induced pluripotent stem (iPS) cells; therefore, identification of chemicals and cooperative factors that may improve the generation of iPS cells will be of great value. Moreover, the cellular mechanisms that limit the reprogramming efficiency need to be investigated. Through screening a chemical library, we found that two chemicals reported to upregulate E-cadherin considerably increase the reprogramming efficiency. Further study of the process indicated that E-cadherin is upregulated during reprogramming and the established iPS cells possess E-cadherin-mediated cell-cell contact, morphologically indistinguishable from embryonic stem (ES) cells. Our experiments also demonstrate that overexpression of E-cadherin significantly enhances reprogramming efficiency, whereas knockdown of endogenous E-cadherin reduces the efficiency. Consistently, abrogation of cell-cell contact by the inhibitory peptide or the neutralizing antibody against the extracellular domain of E-cadherin compromises iPS cell generation. Further mechanistic study reveals that adhesive binding activity of E-cadherin is required. Our results highlight the critical role of E-cadherin-mediated cell-cell contact in reprogramming and suggest new routes for more efficient iPS cell generation. STEM CELLS 2010; 28: 1315-1325 |
| 学科主题 | Cell Biology; Biotechnology & Applied Microbiology; Oncology; Hematology |
| 类目[WOS] | Cell & Tissue Engineering ; Biotechnology & Applied Microbiology ; Oncology ; Cell Biology ; Hematology |
| 关键词[WOS] | SMALL-MOLECULE COMPOUNDS ; SELF-RENEWAL ; DROSOPHILA-OVARY ; PROSTATE-CANCER ; MOUSE ; ADHESION ; FIBROBLASTS ; EXPRESSION ; INDUCTION ; APIGENIN |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000281566200002 |
| 版本 | 出版稿 |
| 源URL | [http://202.127.25.143/handle/331003/1040]  |
| 专题 | 上海生化细胞研究所_上海生科院生化细胞研究所 |
| 推荐引用方式 GB/T 7714 | Chen, TT,Yuan, DT,Wei, B,et al. E-Cadherin-Mediated Cell-Cell Contact Is Critical for Induced Pluripotent Stem Cell Generation[J]. STEM CELLS,2010,28(8):1315-1325. |
| APA | Chen, TT.,Yuan, DT.,Wei, B.,Jiang, J.,Kang, JH.,...&Pei, G.(2010).E-Cadherin-Mediated Cell-Cell Contact Is Critical for Induced Pluripotent Stem Cell Generation.STEM CELLS,28(8),1315-1325. |
| MLA | Chen, TT,et al."E-Cadherin-Mediated Cell-Cell Contact Is Critical for Induced Pluripotent Stem Cell Generation".STEM CELLS 28.8(2010):1315-1325. |
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