Structural Transformation of the Tandem Ubiquitin-Interacting Motifs in Ataxin-3 and Their Cooperative Interactions with Ubiquitin Chains
文献类型：期刊论文
| 作者 | Song, AX; Zhou, CJ; Peng, Y; Gao, XC; Zhou, ZR; Fu, QS; Hong, J; Lin, DH; Hu, HY |
| 刊名 | PLOS ONE
 |
| 出版日期 | 2010 |
| 卷号 | 5期号:10页码:e13202-e13202 |
| 通讯作者 | Song, AX (reprint author), Chinese Acad Sci, State Key Lab Mol Biol, Inst Biochem & Cell Biol, Shanghai Inst Biol Sci, Shanghai, Peoples R China.,dhlin@mail.shcnc.ac.cn ; hyhu@sibs.ac.cn |
| 英文摘要 | The ubiquitin-interacting motif (UIM) is a short peptide with dual function of binding ubiquitin (Ub) and promoting ubiquitination. We elucidated the structures and dynamics of the tandem UIMs of ataxin-3 (AT3-UIM12) both in free and Ub-bound forms. The solution structure of free AT3-UIM12 consists of two alpha-helices and a flexible linker, whereas that of the Ub-bound form is much more compact with hydrophobic contacts between the two helices. NMR dynamics indicates that the flexible linker becomes rigid when AT3-UIM12 binds with Ub. Isothermal titration calorimetry and NMR titration demonstrate that AT3-UIM12 binds diUb with two distinct affinities, and the linker plays a critical role in association of the two helices in diUb binding. These results provide an implication that the tandem UIM12 interacts with Ub or diUb in a cooperative manner through an allosteric effect and dynamics change of the linker region, which might be related to its recognitions with various Ub chains and ubiquitinated substrates. |
| 学科主题 | Science & Technology - Other Topics |
| 类目[WOS] | Multidisciplinary Sciences |
| 关键词[WOS] | POLYGLUTAMINE DISEASE PROTEIN ; DEUBIQUITINATING ENZYME ATAXIN-3 ; MACHADO-JOSEPH-DISEASE ; NMR SOLUTION STRUCTURE ; BINDING DOMAINS ; CRYSTAL-STRUCTURE ; CHEMICAL-SHIFT ; RECOGNITION ; SYSTEM ; PROTEASOME |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000282633700009 |
| 版本 | 出版稿 |
| 源URL | [http://202.127.25.143/handle/331003/1052]  |
| 专题 | 上海生化细胞研究所_上海生科院生化细胞研究所 |
| 推荐引用方式 GB/T 7714 | Song, AX,Zhou, CJ,Peng, Y,et al. Structural Transformation of the Tandem Ubiquitin-Interacting Motifs in Ataxin-3 and Their Cooperative Interactions with Ubiquitin Chains[J]. PLOS ONE,2010,5(10):e13202-e13202. |
| APA | Song, AX.,Zhou, CJ.,Peng, Y.,Gao, XC.,Zhou, ZR.,...&Hu, HY.(2010).Structural Transformation of the Tandem Ubiquitin-Interacting Motifs in Ataxin-3 and Their Cooperative Interactions with Ubiquitin Chains.PLOS ONE,5(10),e13202-e13202. |
| MLA | Song, AX,et al."Structural Transformation of the Tandem Ubiquitin-Interacting Motifs in Ataxin-3 and Their Cooperative Interactions with Ubiquitin Chains".PLOS ONE 5.10(2010):e13202-e13202. |
入库方式： OAI收割
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。