Osteogenic growth peptide enhances the proliferation of bone marrow mesenchymal stem cells from osteoprotegerin-deficient mice by CDK2/cyclin A
文献类型：期刊论文
| 作者 | Fei, QM; Guo, CJ; Xu, XY; Gao, JJ; Zhang, JA; Chen, TY; Cui, DF |
| 刊名 | ACTA BIOCHIMICA ET BIOPHYSICA SINICA
 |
| 出版日期 | 2010 |
| 卷号 | 42期号:11页码:801-806 |
| 关键词 | osteogenic growth peptide osteoprotegerin mesenchymal stem cell cell proliferation CDK2 cyclin A |
| 通讯作者 | Fei, QM (reprint author), Fudan Univ, Zhongshan Hosp, Dept Orthopaed Surg, Shanghai 200032, Peoples R China.,fei.qinming@zs-hospital.sh.cn |
| 英文摘要 | To promote bone formation is one of the fundamental strategies in osteoporosis treatment and fractures repair. As one of the stimulators on bone formation, osteogenic growth peptide (OGP) increases both proliferation and differentiation of the osteoblasts in vitro and in vivo, in which osteoprotegerin (OPG) has been suggested being involved. In this study, we evaluated the effects of OGP on bone marrow mesenchymal stem cells (MSCs) from OPG-deficient mice in vitro by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay, alkaline phosphatase (ALP) activity assay, real-time polymerase chain reaction, and western blot analysis. Results showed that OGP stimulated MSC proliferation and increased the expression of CDK2 and cyclin A in MSCs both at mRNA and protein levels. However, no differentiative effect of OGP was shown as ALP activity and the expression levels of Runx2 and Osterix were not increased significantly by OGP. Our study suggested that OGP may increase the bone formation in OPG-deficient mice by stimulating MSC proliferation rather than differentiation, and probably by triggering CDK2/cyclin A pathway. |
| 学科主题 | Biochemistry & Molecular Biology; Biophysics |
| 类目[WOS] | Biochemistry & Molecular Biology ; Biophysics |
| 关键词[WOS] | STROMAL CELLS ; DIFFERENTIATION ; OSTEOPOROSIS ; CULTURES |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000283657600006 |
| 版本 | 出版稿 |
| 源URL | [http://202.127.25.143/handle/331003/1066]  |
| 专题 | 上海生化细胞研究所_上海生科院生化细胞研究所 |
| 推荐引用方式 GB/T 7714 | Fei, QM,Guo, CJ,Xu, XY,et al. Osteogenic growth peptide enhances the proliferation of bone marrow mesenchymal stem cells from osteoprotegerin-deficient mice by CDK2/cyclin A[J]. ACTA BIOCHIMICA ET BIOPHYSICA SINICA,2010,42(11):801-806. |
| APA | Fei, QM.,Guo, CJ.,Xu, XY.,Gao, JJ.,Zhang, JA.,...&Cui, DF.(2010).Osteogenic growth peptide enhances the proliferation of bone marrow mesenchymal stem cells from osteoprotegerin-deficient mice by CDK2/cyclin A.ACTA BIOCHIMICA ET BIOPHYSICA SINICA,42(11),801-806. |
| MLA | Fei, QM,et al."Osteogenic growth peptide enhances the proliferation of bone marrow mesenchymal stem cells from osteoprotegerin-deficient mice by CDK2/cyclin A".ACTA BIOCHIMICA ET BIOPHYSICA SINICA 42.11(2010):801-806. |
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