Conformational and biochemical characterization of a rat epididymis-specific lipocalin 12 expressed in Escherichia coli
文献类型：期刊论文
| 作者 | Peng, Y; Liu, JF; Liu, QA; Yao, YH; Guo, CY; Zhang, YL; Lin, DH |
| 刊名 | BIOCHIMICA ET BIOPHYSICA ACTA-PROTEINS AND PROTEOMICS
 |
| 出版日期 | 2010 |
| 卷号 | 1804期号:11页码:2102-2110 |
| 关键词 | Lipocalin 12 (Lcn12) Epididymis beta-Barrel Cysteine Disulfide bond Retinoic acid |
| 通讯作者 | Lin, DH (reprint author), Chinese Acad Sci, Shanghai Inst Mat Med, Lab Biomol NMR, Shanghai 201203, Peoples R China.,dhlin@mail.shcnc.ac.cn |
| 英文摘要 | Lipocalin 12 (Lcn12) is a recently identified epididymis-specific protein that might play a significant physiological role in male reproduction. However, the detailed structure and function of Lcn12 remain to be determined. In the present work, we cloned, expressed, and purified the rat Lcn12 (rLcn12) protein in Escherichia coli, introduced the Cys176Ala substitution to eliminate the aggregation problem associated with the wild-type protein. Homology modeling results demonstrated that rLcn12 adopted an eight-stranded, antiparallel beta-barrel conformation containing a conserved disulfide bond between Cys98 and Cys203, which was in accordance with the physicochemical properties elucidated by a combination of mass, circular dichroism, and nuclear magnetic resonance spectrometry. The purified rLcn12 protein exhibited a high binding affinity for all-trans retinoic acid in fluorescence titration experiments, implying that rLcn12 could be involved in retinoic acid transport in the epididymis. (C) 2010 Elsevier B.V. All rights reserved. |
| 学科主题 | Biochemistry & Molecular Biology; Biophysics |
| 类目[WOS] | Biochemistry & Molecular Biology ; Biophysics |
| 关键词[WOS] | ACID-BINDING PROTEIN ; CONSERVED DISULFIDE BOND ; PROSTAGLANDIN-D SYNTHASE ; LIGAND-BINDING ; FAMILY ; SEQUENCE ; NGAL ; STABILITY ; EVOLUTION ; RETINOIDS |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000282720700002 |
| 版本 | 出版稿 |
| 源URL | [http://202.127.25.143/handle/331003/1067]  |
| 专题 | 上海生化细胞研究所_上海生科院生化细胞研究所 |
| 推荐引用方式 GB/T 7714 | Peng, Y,Liu, JF,Liu, QA,et al. Conformational and biochemical characterization of a rat epididymis-specific lipocalin 12 expressed in Escherichia coli[J]. BIOCHIMICA ET BIOPHYSICA ACTA-PROTEINS AND PROTEOMICS,2010,1804(11):2102-2110. |
| APA | Peng, Y.,Liu, JF.,Liu, QA.,Yao, YH.,Guo, CY.,...&Lin, DH.(2010).Conformational and biochemical characterization of a rat epididymis-specific lipocalin 12 expressed in Escherichia coli.BIOCHIMICA ET BIOPHYSICA ACTA-PROTEINS AND PROTEOMICS,1804(11),2102-2110. |
| MLA | Peng, Y,et al."Conformational and biochemical characterization of a rat epididymis-specific lipocalin 12 expressed in Escherichia coli".BIOCHIMICA ET BIOPHYSICA ACTA-PROTEINS AND PROTEOMICS 1804.11(2010):2102-2110. |
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