Identification of a Smad4/YY1-Recognized and BMP2-Responsive Transcriptional Regulatory Module in the Promoter of Mouse GABA Transporter Subtype I (Gat1) Gene
文献类型：期刊论文
| 作者 | Yao, MH; Niu, G; Sheng, ZJ; Wang, ZG; Fei, J |
| 刊名 | JOURNAL OF NEUROSCIENCE
 |
| 出版日期 | 2010 |
| 卷号 | 30期号:11页码:4062-4071 |
| 通讯作者 | Fei, J (reprint author), Tongji Univ, Sch Life Sci & Technol, 1239 Siping Rd, Shanghai 200092, Peoples R China.,jfei@mail.tongji.edu.cn |
| 英文摘要 | GABAergic dysfunction is implicated in a variety of neurodevelopmental and psychiatric disorders. The mechanisms underlying GABAergic differentiation, however, are not well understood. GABA transporter 1 (Gat1; Slc6a1) is an essential component of the GABAergic system, and its ectopic mRNA expression may be responsible for GABAergic malfunction under different pathological conditions. Thus, monitoring the transcriptional regulation of gat1 may help to elucidate the mechanisms that govern the differentiation of GABAergic neurons. In this study, we identified a promoter region that is sufficient to recapitulate endogenous gat1 expression in transgenic mice. A 46 bp cis-regulator in the promoter sequence was responsible for the stimulation of bone morphogenetic protein-2 (BMP2) on gat1 expression in cortical cortex. Furthermore, our study demonstrated that Smad4 and YY1 are physically bound to the element and mediate both the negative and positive regulatory effects in which BMP2 can affect the balance. In summary, we have identified a Smad4/YY1-based bidirectional regulation model for GABAergic gene transcription and demonstrated a molecular cue important for the differentiation of GABAergic neurons. |
| 学科主题 | Neurosciences & Neurology |
| 类目[WOS] | Neurosciences |
| 关键词[WOS] | DORSAL TELENCEPHALIC PROGENITORS ; CORTICAL GABAERGIC NEURONS ; NEURAL STEM-CELLS ; TRANSGENIC MICE ; NERVOUS-SYSTEM ; FACTOR YY1 ; MESENCHYMAL TRANSITION ; PREFRONTAL CORTEX ; INDUCED SEIZURE ; NEUROPEPTIDE-Y |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000275724800022 |
| 版本 | 出版稿 |
| 源URL | [http://202.127.25.143/handle/331003/1072]  |
| 专题 | 上海生化细胞研究所_上海生科院生化细胞研究所 |
| 推荐引用方式 GB/T 7714 | Yao, MH,Niu, G,Sheng, ZJ,et al. Identification of a Smad4/YY1-Recognized and BMP2-Responsive Transcriptional Regulatory Module in the Promoter of Mouse GABA Transporter Subtype I (Gat1) Gene[J]. JOURNAL OF NEUROSCIENCE,2010,30(11):4062-4071. |
| APA | Yao, MH,Niu, G,Sheng, ZJ,Wang, ZG,&Fei, J.(2010).Identification of a Smad4/YY1-Recognized and BMP2-Responsive Transcriptional Regulatory Module in the Promoter of Mouse GABA Transporter Subtype I (Gat1) Gene.JOURNAL OF NEUROSCIENCE,30(11),4062-4071. |
| MLA | Yao, MH,et al."Identification of a Smad4/YY1-Recognized and BMP2-Responsive Transcriptional Regulatory Module in the Promoter of Mouse GABA Transporter Subtype I (Gat1) Gene".JOURNAL OF NEUROSCIENCE 30.11(2010):4062-4071. |
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