Involvement of Cenp-F in interphase chromatin organization possibly through association with DNA-dependent protein kinase
文献类型：期刊论文
| 作者 | Du, JA; Zhang, YR; Liu, Y; Li, Y; Zhu, XL |
| 刊名 | ACTA BIOCHIMICA ET BIOPHYSICA SINICA
 |
| 出版日期 | 2010 |
| 卷号 | 42期号:12页码:839-846 |
| 关键词 | Cenp-F chromatin organization DNA-PK mitosin nuclear matrix |
| 通讯作者 | Zhu, XL (reprint author), Chinese Acad Sci, Mol Cell Biol Lab, Inst Biochem & Cell Biol, Shanghai Inst Biol Sci, Shanghai 200031, Peoples R China.,xlzhu@sibs.ac.cn |
| 英文摘要 | Cenp-F (also named mitosin) is a 350-kDa human kinetochore protein important for the mitotic progression. It is also a nuclear matrix protein in interphase cells. Here, we showed that overexpression of N-terminal deletion mutants of Cenp-F containing the C-terminal 112 residues induced chromatin condensation into numerous aggregates of varying sizes in interphase nucleus, colocalizing with the exogenous proteins. In situ hybridization using whole chromosome painting probes indicated that the chromatin aggregates were not prematurely condensed individual chromosomes. Neither were they due to apoptosis. We provided evidence showing association of Cenp-F with certain regions of interphase chromatin fibers. Cenp-F associated with the DNA-dependent protein kinase (DNA-PK), a trimeric protein complex critical for genome homeostasis. Moreover, the DNA-PK association activity of Cenp-F mutants correlated with their ability to induce chromatin aggregation. These results imply a role of Cenp-F in organization of interphase chromatin through association and possibly regulation of DNA-PK. |
| 学科主题 | Biochemistry & Molecular Biology; Biophysics |
| 类目[WOS] | Biochemistry & Molecular Biology ; Biophysics |
| 关键词[WOS] | NUCLEAR-MATRIX ; KINETOCHORE PROTEIN ; CYTOPLASMIC DYNEIN ; MITOSIN/CENP-F ; HISTONE H3 ; LOCALIZATION ; PHOSPHORYLATION ; CHECKPOINT ; APOPTOSIS ; TERMINUS |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000284634100001 |
| 版本 | 出版稿 |
| 源URL | [http://202.127.25.143/handle/331003/1075]  |
| 专题 | 上海生化细胞研究所_上海生科院生化细胞研究所 |
| 推荐引用方式 GB/T 7714 | Du, JA,Zhang, YR,Liu, Y,et al. Involvement of Cenp-F in interphase chromatin organization possibly through association with DNA-dependent protein kinase[J]. ACTA BIOCHIMICA ET BIOPHYSICA SINICA,2010,42(12):839-846. |
| APA | Du, JA,Zhang, YR,Liu, Y,Li, Y,&Zhu, XL.(2010).Involvement of Cenp-F in interphase chromatin organization possibly through association with DNA-dependent protein kinase.ACTA BIOCHIMICA ET BIOPHYSICA SINICA,42(12),839-846. |
| MLA | Du, JA,et al."Involvement of Cenp-F in interphase chromatin organization possibly through association with DNA-dependent protein kinase".ACTA BIOCHIMICA ET BIOPHYSICA SINICA 42.12(2010):839-846. |
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