Development of retinol-binding protein 4 immunocolloidal gold fast test strip using high-sensitivity monoclonal antibodies generated by DNA immunization
文献类型：期刊论文
| 作者 | Bian, C; Zhang, F; Wang, F; Ling, ZY; Luo, M; Wu, HQ; Sun, YZ; Li, JH; Li, BN; Zhu, JY |
| 刊名 | ACTA BIOCHIMICA ET BIOPHYSICA SINICA
 |
| 出版日期 | 2010 |
| 卷号 | 42期号:12页码:847-853 |
| 关键词 | DNA immunization monoclonal antibody retinol-binding protein 4 immunocolloidal gold renal damage |
| 通讯作者 | Bian, C (reprint author), Chinese Acad Sci, Key Lab Mol Cell Biol, Inst Biochem & Cell Biol, Shanghai Inst Biol Sci, Shanghai 200031, Peoples R China.,cbian@sibs.ac.cn ; bsun@sibs.ac.cn |
| 英文摘要 | DNA immunization is an efficient method for high-affinity monoclonal antibody generation. Here, we describe the generation of several high-quality monoclonal antibodies (mAbs) against retinol-binding protein 4 (RBP4), an important marker for kidney abnormality and dysfunction, with a combination method of DNA priming and protein boost. The mAbs generated could bind to RBP4 with high sensitivity and using these mAbs, an immunocolloidal gold fast test strip was constructed. The strip can give a result in < 5 min and is very sensitive with a detection limit of about 1 ng/ml. A small-scale clinical test revealed that the result of this strip was well in accordance with that of an enzyme-labeled immunosorbent assay kit currently available on the market. Consequently, it could be useful for more convenient and faster RBP4 determination in the clinic. |
| 学科主题 | Biochemistry & Molecular Biology; Biophysics |
| 类目[WOS] | Biochemistry & Molecular Biology ; Biophysics |
| 关键词[WOS] | PLASMA |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000284634100002 |
| 版本 | 出版稿 |
| 源URL | [http://202.127.25.143/handle/331003/1076]  |
| 专题 | 上海生化细胞研究所_上海生科院生化细胞研究所 |
| 推荐引用方式 GB/T 7714 | Bian, C,Zhang, F,Wang, F,et al. Development of retinol-binding protein 4 immunocolloidal gold fast test strip using high-sensitivity monoclonal antibodies generated by DNA immunization[J]. ACTA BIOCHIMICA ET BIOPHYSICA SINICA,2010,42(12):847-853. |
| APA | Bian, C.,Zhang, F.,Wang, F.,Ling, ZY.,Luo, M.,...&Sun, B.(2010).Development of retinol-binding protein 4 immunocolloidal gold fast test strip using high-sensitivity monoclonal antibodies generated by DNA immunization.ACTA BIOCHIMICA ET BIOPHYSICA SINICA,42(12),847-853. |
| MLA | Bian, C,et al."Development of retinol-binding protein 4 immunocolloidal gold fast test strip using high-sensitivity monoclonal antibodies generated by DNA immunization".ACTA BIOCHIMICA ET BIOPHYSICA SINICA 42.12(2010):847-853. |
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