Beneficial effects of C-peptide on renal morphology in diabetic rats
文献类型：期刊论文
| 作者 | Sun, W; Gao, X; Zhao, XL; Cui, DF; Xia, QC |
| 刊名 | ACTA BIOCHIMICA ET BIOPHYSICA SINICA
 |
| 出版日期 | 2010 |
| 卷号 | 42期号:12页码:893-899 |
| 关键词 | C-peptide mesangial matrix type IV collagen diabetic rat mesangial cell |
| 通讯作者 | Gao, X (reprint author), Fudan Univ, Dept Endocrinol, Zhongshan Hosp, Shanghai 200032, Peoples R China.,gao.xin@zs-hospital.sh.cn |
| 英文摘要 | The study was undertaken to examine the effects of C-peptide on glomerular volume (V(GLOM)), mesangial matrix synthesis, and degradation in streptozotocin (STZ)-diabetic rats with poor or moderate glycemic control. Series 1 (poor glycemic control) included groups of healthy rats, hyperglycemic rats, diabetic insulin-treated rats and diabetic C-peptide-treated rats. Series 2 (moderate glycemic control) included groups of healthy rats, diabetic insulin-treated rats, diabetic insulin- and C-peptide-treated rats. After 8 weeks, the left kidney was excised for evaluation of V(GLOM) and mesangial matrix area via light microscopy. Mesangial cells were cultured for 48 h and type IV collagen expression and matrix metalloproteinase (MMP)-2 expression were measured by ELISA and RT-PCR. The results indicated that in Series 1, C-peptide administration suppressed the diabetes-induced increase in the V(GLOM) and the mesangial matrix area. In Series 2, C-peptide administration resulted in a similar decrease in the V(GLOM) and a greater decrease in the mesangial matrix area when compared with insulin therapy alone. Moreover, C-peptide (300 nM) completely inhibited the glucose-induced increase of the collagen IV mRNA expression and protein concentration in mesangial cells cultured in 30 mM glucose medium. MMP-2 mRNA expression was not influenced by C-peptide. In conclusion, C-peptide administration to STZ-diabetic rats for 8 weeks results in the inhibition of diabetes-induced expansion of the mesangial matrix. This effect is independent of the level of glycemic control and results from the inhibition of diabetes-induced excessive formation of mesangial type IV collagen. |
| 学科主题 | Biochemistry & Molecular Biology; Biophysics |
| 类目[WOS] | Biochemistry & Molecular Biology ; Biophysics |
| 关键词[WOS] | MATRIX METALLOPROTEINASES ; TYPE-1 ; NEPHROPATHY ; MELLITUS |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000284634100007 |
| 版本 | 出版稿 |
| 源URL | [http://202.127.25.143/handle/331003/1077]  |
| 专题 | 上海生化细胞研究所_上海生科院生化细胞研究所 |
| 推荐引用方式 GB/T 7714 | Sun, W,Gao, X,Zhao, XL,et al. Beneficial effects of C-peptide on renal morphology in diabetic rats[J]. ACTA BIOCHIMICA ET BIOPHYSICA SINICA,2010,42(12):893-899. |
| APA | Sun, W,Gao, X,Zhao, XL,Cui, DF,&Xia, QC.(2010).Beneficial effects of C-peptide on renal morphology in diabetic rats.ACTA BIOCHIMICA ET BIOPHYSICA SINICA,42(12),893-899. |
| MLA | Sun, W,et al."Beneficial effects of C-peptide on renal morphology in diabetic rats".ACTA BIOCHIMICA ET BIOPHYSICA SINICA 42.12(2010):893-899. |
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