Research Resource: Genome-Wide Mapping of in Vivo Androgen Receptor Binding Sites in Mouse Epididymis
文献类型：期刊论文
| 作者 | Hu, SG; Yao, GX; Guan, XJ; Ni, ZM; Ma, WB; Wilson, EM; French, FS; Liu, QA; Zhang, YL |
| 刊名 | MOLECULAR ENDOCRINOLOGY
 |
| 出版日期 | 2010 |
| 卷号 | 24期号:12页码:2392-2405 |
| 通讯作者 | Zhang, YL (reprint author), Chinese Acad Sci, Shanghai Inst Biol Sci, Inst Biochem & Cell Biol, State Key Lab Mol Biol,Shanghai Key Lab Mol Andro, Shanghai 20031, Peoples R China.,qliu@sibs.ac.cn ; ylzhang@sibs.ac.cn |
| 英文摘要 | Epididymal function depends on androgen signaling through the androgen receptor (AR), although most of the direct AR target genes in epididymis remain unknown. Here we globally mapped the AR binding regions in mouse caput epididymis in which AR is highly expressed. Chromatin immunoprecipitation sequencing indicated that AR bound selectively to 19,377 DNA regions, the majority of which were intergenic and intronic. Motif analysis showed that 94% of the AR binding regions harbored consensus androgen response elements enriched with multiple binding motifs that included nuclear factor 1 and activator protein 2 sites consistent with combinatorial regulation. Unexpectedly, AR binding regions showed limited conservation across species, regardless of whether the metric for conservation was based on local sequence similarity or the presence of consensus androgen response elements. Further analysis suggested the AR target genes are involved in diverse biological themes that include lipid metabolism and sperm maturation. Potential novel mechanisms of AR regulation were revealed at individual genes such as cysteine-rich secretory protein 1. The composite studies provide new insights into AR regulation under physiological conditions and a global resource of AR binding sites in a normal androgen-responsive tissue. (Molecular Endocrinology 24: 2392-2405, 2010) |
| 学科主题 | Endocrinology & Metabolism |
| 类目[WOS] | Endocrinology & Metabolism |
| 关键词[WOS] | PROSTATE-CANCER GROWTH ; TRANSCRIPTION FACTOR ; RESPONSE ELEMENTS ; GENE-EXPRESSION ; AR COREGULATORS ; CELLS ; DNA ; IDENTIFICATION ; MICROARRAY ; PROTEINS |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000284585200013 |
| 版本 | 出版稿 |
| 源URL | [http://202.127.25.143/handle/331003/1080]  |
| 专题 | 上海生化细胞研究所_上海生科院生化细胞研究所 |
| 推荐引用方式 GB/T 7714 | Hu, SG,Yao, GX,Guan, XJ,et al. Research Resource: Genome-Wide Mapping of in Vivo Androgen Receptor Binding Sites in Mouse Epididymis[J]. MOLECULAR ENDOCRINOLOGY,2010,24(12):2392-2405. |
| APA | Hu, SG.,Yao, GX.,Guan, XJ.,Ni, ZM.,Ma, WB.,...&Zhang, YL.(2010).Research Resource: Genome-Wide Mapping of in Vivo Androgen Receptor Binding Sites in Mouse Epididymis.MOLECULAR ENDOCRINOLOGY,24(12),2392-2405. |
| MLA | Hu, SG,et al."Research Resource: Genome-Wide Mapping of in Vivo Androgen Receptor Binding Sites in Mouse Epididymis".MOLECULAR ENDOCRINOLOGY 24.12(2010):2392-2405. |
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