Distinct functions of BMP4 during different stages of mouse ES cell neural commitment
文献类型：期刊论文
| 作者 | Zhang, KJ; Li, LY; Huang, CY; Shen, CY; Tan, FZ; Xia, CH; Liu, PY; Rossant, J; Jing, NH |
| 刊名 | DEVELOPMENT
 |
| 出版日期 | 2010 |
| 卷号 | 137期号:13页码:2095-2105 |
| 关键词 | Embryonic stem cells Epiblasts EpiSCs BMP4 Neural commitment Mouse |
| 通讯作者 | Jing, NH (reprint author), Chinese Acad Sci, Shanghai Inst Biol Sci, Inst Biochem & Cell Biol, Mol Cell Biol Lab, 320 Yue Yang Rd, Shanghai 200031, Peoples R China.,njing@sibs.ac.cn |
| 英文摘要 | Bone morphogenetic protein (BMP) signaling plays a crucial role in maintaining the pluripotency of mouse embryonic stem cells (ESCs) and has negative effects on ESC neural differentiation. However, it remains unclear when and how BMP signaling executes those different functions during neural commitment. Here, we show that a BMP4-sensitive window exists during ESC neural differentiation. Cells at this specific period correspond to the egg cylinder stage epiblast and can be maintained as ESC-derived epiblast stem cells (ESD-EpiSCs), which have the same characteristics as EpiSCs derived from mouse embryos. We propose that ESC neural differentiation occurs in two stages: first from ESCs to ESD-EpiSCs and then from ESD-EpiSCs to neural precursor cells (NPCs). We further show that BMP4 inhibits the conversion of ESCs into ESD-EpiSCs during the first stage, and suppresses ESD-EpiSC neural commitment and promotes non-neural lineage differentiation during the second stage. Mechanistic studies show that BMP4 inhibits FGF/ERK activity at the first stage but not at the second stage; and IDs, as important downstream genes of BMP signaling, partially substitute for BMP4 functions at both stages. We conclude that BMP signaling has distinct functions during different stages of ESC neural commitment. |
| 学科主题 | Developmental Biology |
| 类目[WOS] | Developmental Biology |
| 关键词[WOS] | EMBRYONIC STEM-CELLS ; SELF-RENEWAL ; MAMMALIAN EMBRYO ; TRANSCRIPTION FACTOR ; GROUND-STATE ; INDUCTION ; GENE ; EXPRESSION ; PLURIPOTENCY ; GASTRULATION |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000278559900003 |
| 版本 | 出版稿 |
| 源URL | [http://202.127.25.143/handle/331003/1082]  |
| 专题 | 上海生化细胞研究所_上海生科院生化细胞研究所 |
| 推荐引用方式 GB/T 7714 | Zhang, KJ,Li, LY,Huang, CY,et al. Distinct functions of BMP4 during different stages of mouse ES cell neural commitment[J]. DEVELOPMENT,2010,137(13):2095-2105. |
| APA | Zhang, KJ.,Li, LY.,Huang, CY.,Shen, CY.,Tan, FZ.,...&Jing, NH.(2010).Distinct functions of BMP4 during different stages of mouse ES cell neural commitment.DEVELOPMENT,137(13),2095-2105. |
| MLA | Zhang, KJ,et al."Distinct functions of BMP4 during different stages of mouse ES cell neural commitment".DEVELOPMENT 137.13(2010):2095-2105. |
入库方式： OAI收割
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。