Deficiency in a Glutamine-Specific Methyltransferase for Release Factor Causes Mouse Embryonic Lethality
文献类型：期刊论文
| 作者 | Liu, P; Nie, S; Li, B; Yang, ZQ; Xu, ZM; Fei, JA; Lin, CS; Zeng, R; Xu, GL |
| 刊名 | MOLECULAR AND CELLULAR BIOLOGY
 |
| 出版日期 | 2010 |
| 卷号 | 30期号:17页码:4245-4253 |
| 通讯作者 | Xu, GL (reprint author), Chinese Acad Sci, Shanghai Inst Biol Sci, Inst Biochem & Cell Biol, State Key Lab Mol Biol, 320 Yueyang Rd, Shanghai 200031, Peoples R China.,glxu@sibs.ac.cn |
| 英文摘要 | Biological methylation is a fundamental enzymatic reaction for a variety of substrates in multiple cellular processes. Mammalian N6amt1 was thought to be a homologue of bacterial N(6)-adenine DNA methyltransferases, but its substrate specificity and physiological importance remain elusive. Here, we demonstrate that N6amt1 functions as a protein methyltransferase for the translation termination factor eRF1 in mammalian cells both in vitro and in vivo. Mass spectrometry analysis indicated that about 70% of the endogenous eRF1 is methylated at the glutamine residue of the conserved GGQ motif. To address the physiological significance of eRF1 methylation, we disrupted the N6amt1 gene in the mouse. Loss of N6amt1 led to early embryonic lethality. The postimplantation development of mutant embryos was impaired, resulting in degeneration around embryonic day 6.5. This is in contrast to what occurs in Escherichia coli and Saccharomyces cerevisiae, which can survive without the N6amt1 homologues. Thus, N6amt1 is the first glutamine-specific protein methyltransferase characterized in vivo in mammals and methylation of eRF1 by N6amt1 might be essential for the viability of early embryos. |
| 学科主题 | Biochemistry & Molecular Biology; Cell Biology |
| 类目[WOS] | Biochemistry & Molecular Biology ; Cell Biology |
| 关键词[WOS] | ESCHERICHIA-COLI ; FACTOR ERF1 ; GGQ MOTIF ; IN-VIVO ; PROTEIN ; DNA ; GENE ; TRANSLATION ; TERMINATION ; HEMK |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000280762900013 |
| 版本 | 出版稿 |
| 源URL | [http://202.127.25.143/handle/331003/1083]  |
| 专题 | 上海生化细胞研究所_上海生科院生化细胞研究所 |
| 推荐引用方式 GB/T 7714 | Liu, P,Nie, S,Li, B,et al. Deficiency in a Glutamine-Specific Methyltransferase for Release Factor Causes Mouse Embryonic Lethality[J]. MOLECULAR AND CELLULAR BIOLOGY,2010,30(17):4245-4253. |
| APA | Liu, P.,Nie, S.,Li, B.,Yang, ZQ.,Xu, ZM.,...&Xu, GL.(2010).Deficiency in a Glutamine-Specific Methyltransferase for Release Factor Causes Mouse Embryonic Lethality.MOLECULAR AND CELLULAR BIOLOGY,30(17),4245-4253. |
| MLA | Liu, P,et al."Deficiency in a Glutamine-Specific Methyltransferase for Release Factor Causes Mouse Embryonic Lethality".MOLECULAR AND CELLULAR BIOLOGY 30.17(2010):4245-4253. |
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