Studying base pair open-close kinetics of tRNA(Leu) by TROSY-based proton exchange NMR spectroscopy
文献类型：期刊论文
| 作者 | Hao, ZX; Tan, M; Liu, CD; Feng, R; Wang, ED; Zhu, G |
| 刊名 | FEBS LETTERS
 |
| 出版日期 | 2010 |
| 卷号 | 584期号:21页码:4449-4452 |
| 关键词 | tRNA(Leu) Nuclear magnetic resonance Proton exchange Base pair open-close kinetic Millisecond conformational flexibility Aminoacylation |
| 通讯作者 | Wang, ED (reprint author), Chinese Acad Sci, Grad Sch, Shanghai Inst Biol Sci, State Key Lab Mol Biol,Inst Biochem & Cell Biol, 320 Yueyang Rd, Shanghai 200031, Peoples R China.,edwang@sibs.ac.cn ; gzhu@ust.hk |
| 英文摘要 | The millisecond conformational flexibility is functionally important for nucleic acids and can be studied through probing the base pair open-close kinetics by proton exchange nuclear magnetic resonance (NMR) spectroscopy. Here, the traditional imino proton exchange NMR experiments were modified with transverse relaxation optimized spectroscopy and were applied to accurately measure imino proton exchange rates of all base pairs in Escherichia coli tRNA(Leu) (CAG), and their dependence on magnesium ion concentration. Finally, we correlated millisecond conformational flexibility with aminoacylation of tRNA(Leu) and proposed that the flexibility of the acceptor stem and the core region might contribute to aminoacylation of tRNA(Leu). (C) 2010 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved. |
| 学科主题 | Biochemistry & Molecular Biology; Biophysics; Cell Biology |
| 类目[WOS] | Biochemistry & Molecular Biology ; Biophysics ; Cell Biology |
| 关键词[WOS] | ESCHERICHIA-COLI TRNA(LEU) ; TRANSFER-RNA SYNTHETASES ; AMINOACYLATION ; PURIFICATION ; RELAXATION ; IDENTITY ; HSQC ; SETS |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000283573100013 |
| 版本 | 出版稿 |
| 源URL | [http://202.127.25.143/handle/331003/1088]  |
| 专题 | 上海生化细胞研究所_上海生科院生化细胞研究所 |
| 推荐引用方式 GB/T 7714 | Hao, ZX,Tan, M,Liu, CD,et al. Studying base pair open-close kinetics of tRNA(Leu) by TROSY-based proton exchange NMR spectroscopy[J]. FEBS LETTERS,2010,584(21):4449-4452. |
| APA | Hao, ZX,Tan, M,Liu, CD,Feng, R,Wang, ED,&Zhu, G.(2010).Studying base pair open-close kinetics of tRNA(Leu) by TROSY-based proton exchange NMR spectroscopy.FEBS LETTERS,584(21),4449-4452. |
| MLA | Hao, ZX,et al."Studying base pair open-close kinetics of tRNA(Leu) by TROSY-based proton exchange NMR spectroscopy".FEBS LETTERS 584.21(2010):4449-4452. |
入库方式： OAI收割
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。