In vitro production of haploid sperm cells from male germ cells of foetal cattle
文献类型：期刊论文
| 作者 | Dong, WZ; Hua, JL; Shen, WZ; Dou, ZY |
| 刊名 | ANIMAL REPRODUCTION SCIENCE
 |
| 出版日期 | 2010 |
| 卷号 | 118期号:1页码:103-109 |
| 关键词 | Foetal cattle Male germ cells Retinol acid (RA) Haploid sperm cells |
| 通讯作者 | Dong, WZ (reprint author), NW A&F Univ, Inst Stem Cell Engn & Technol, Yangling 712100, Peoples R China.,wzdong@sibs.ac.cn |
| 英文摘要 | The purpose of this study was to isolate the foetal cattle male germ cells (mGCs) and then induce them into sperm cells. The mGCs were purified and enriched by a two-step plating method based on the different adherence velocities of mGCs and somatic cells. The percentage of the vasa and the c-kit positive cells were 95.34 +/- 2.25% and 53.3 +/- 1.03% by using flow cytometry analysis (FCA), respectively. In feeder-free culture system, the half-suspending cells appeared and formed a 16-cell rosary in medium after the mGCs were cultured for 6-8 days. On immunocytochemical staining during the second passage, some single cells adhering to the plate appeared to be both Oct-4 and alpha 6-integrin positive. During the third passage, the mGCs were induced for 48 h by retinol acid (RA) on Sertoli cell-feeder layer, followed by 5-7 days culture in an RA-free medium. Some elongated sperm-like cells appeared in the medium at this stage. We found that the most effective concentration of RA for the inducement was 10(-7) mol l(-1) (P<0.01). The haploid cells in suspension were identified by FCA. The elongated sperm-like cells showed proacrosome-like structure and the flagellum with fibre construct under electron microscopy. The mRNA of outer dense fibre-3 (ODF-3) and transcription protein-1 (TP-1) could be detected in the suspended cells by using reverse transcription polymerase chain reaction (RT-PCR). About 23.1% bovine oocytes could be activated to perform cleavage by intracytoplasmic injection with the sperm-like cells, but embryos did not further develop. Our investigation further demonstrated that foetal cattle mGCs could be induced in vitro into haploid sperm in the short term. (C) 2009 Elsevier B.V. All rights reserved. |
| 学科主题 | Agriculture ; Reproductive Biology |
| 类目[WOS] | Agriculture, Dairy & Animal Science ; Reproductive Biology |
| 关键词[WOS] | LONG-TERM CULTURE ; SPERMATOGONIAL STEM-CELLS ; RETINOIC-ACID ; C-KIT ; A SPERMATOGONIA ; SURFACE-MARKER ; DIFFERENTIATION ; MOUSE ; MICE ; PROLIFERATION |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000274758600001 |
| 版本 | 出版稿 |
| 源URL | [http://202.127.25.143/handle/331003/1102]  |
| 专题 | 上海生化细胞研究所_上海生科院生化细胞研究所 |
| 推荐引用方式 GB/T 7714 | Dong, WZ,Hua, JL,Shen, WZ,et al. In vitro production of haploid sperm cells from male germ cells of foetal cattle[J]. ANIMAL REPRODUCTION SCIENCE,2010,118(1):103-109. |
| APA | Dong, WZ,Hua, JL,Shen, WZ,&Dou, ZY.(2010).In vitro production of haploid sperm cells from male germ cells of foetal cattle.ANIMAL REPRODUCTION SCIENCE,118(1),103-109. |
| MLA | Dong, WZ,et al."In vitro production of haploid sperm cells from male germ cells of foetal cattle".ANIMAL REPRODUCTION SCIENCE 118.1(2010):103-109. |
入库方式： OAI收割
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。