Proteomics analysis of serum protein profiling in pancreatic cancer patients by DIGE: up-regulation of mannose-binding lectin 2 and myosin light chain kinase 2
文献类型：期刊论文
| 作者 | Rong, YF; Jin, DY; Hou, CR; Hu, JW; Wu, WC; Ni, XL; Wang, DS; Lou, WH |
| 刊名 | BMC GASTROENTEROLOGY
 |
| 出版日期 | 2010 |
| 卷号 | 10期号:1页码:68-68 |
| 通讯作者 | Lou, WH (reprint author), Fudan Univ, Dept Gen Surg, Pancreat Canc Grp, Zhongshan Hosp, Shanghai 200032, Peoples R China.,wenhuilou@yahoo.com.cn |
| 英文摘要 | Background: Pancreatic cancer has significant morbidity and mortality worldwide. Good prognosis relies on an early diagnosis. The purpose of this study was to develop techniques for identifying cancer biomarkers in the serum of patients with pancreatic cancer. Methods: Serum samples from five individuals with pancreatic cancer and five individuals without cancer were compared. Highly abundant serum proteins were depleted by immuno-affinity column. Differential protein analysis was performed using 2-dimensional differential in-gel electrophoresis (2D-DIGE). Results: Among these protein spots, we found that 16 protein spots were differently expressed between the two mixtures; 8 of these were up-regulated and 8 were down-regulated in cancer. Mass spectrometry and database searching allowed the identification of the proteins corresponding to the gel spots. Up-regulation of mannose-binding lectin 2 and myosin light chain kinase 2, which have not previously been implicated in pancreatic cancer, were observed. In an independent series of serum samples from 16 patients with pancreatic cancer and 16 non-cancer-bearing controls, increased levels of mannose-binding lectin 2 and myosin light chain kinase 2 were confirmed by western blot. Conclusions: These results suggest that affinity column enrichment and DIGE can be used to identify proteins differentially expressed in serum from pancreatic cancer patients. These two proteins 'mannose-binding lectin 2 and myosin light chain kinase 2' might be potential biomarkers for the diagnosis of the pancreatic cancer. |
| 学科主题 | Gastroenterology & Hepatology |
| 类目[WOS] | Gastroenterology & Hepatology |
| 关键词[WOS] | INNATE-IMMUNITY ; BREAST-CANCER ; LUNG-CANCER ; DIAGNOSIS ; CELLS ; CA19-9 ; MBL2 ; POLYMORPHISMS ; CONTRIBUTES ; ACTIVATION |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000280394900001 |
| 版本 | 出版稿 |
| 源URL | [http://202.127.25.143/handle/331003/1104]  |
| 专题 | 上海生化细胞研究所_上海生科院生化细胞研究所 |
| 推荐引用方式 GB/T 7714 | Rong, YF,Jin, DY,Hou, CR,et al. Proteomics analysis of serum protein profiling in pancreatic cancer patients by DIGE: up-regulation of mannose-binding lectin 2 and myosin light chain kinase 2[J]. BMC GASTROENTEROLOGY,2010,10(1):68-68. |
| APA | Rong, YF.,Jin, DY.,Hou, CR.,Hu, JW.,Wu, WC.,...&Lou, WH.(2010).Proteomics analysis of serum protein profiling in pancreatic cancer patients by DIGE: up-regulation of mannose-binding lectin 2 and myosin light chain kinase 2.BMC GASTROENTEROLOGY,10(1),68-68. |
| MLA | Rong, YF,et al."Proteomics analysis of serum protein profiling in pancreatic cancer patients by DIGE: up-regulation of mannose-binding lectin 2 and myosin light chain kinase 2".BMC GASTROENTEROLOGY 10.1(2010):68-68. |
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