Identification of galectin-7 as a potential biomarker for esophageal squamous cell carcinoma by proteomic analysis
文献类型：期刊论文
| 作者 | Zhu, X; Ding, M; Yu, ML; Feng, MX; Tan, LJ; Zhao, FK |
| 刊名 | BMC CANCER
 |
| 出版日期 | 2010 |
| 卷号 | 10期号:1页码:290-290 |
| 通讯作者 | Feng, MX (reprint author), Fu Dan Univ, Zhong Shan Hosp, Dept Thorac Surg, Shanghai 200032, Peoples R China.,feng.mingxiang@zs-hospital.sh.cn ; fkzhao@sibs.ac.cn |
| 英文摘要 | Background: Esophageal squamous cell carcinoma (ESCC) is one of the most common malignancies. Early diagnosis is critical for guiding the therapeutic management of ESCC. It is imperative to find more effective biomarkers of ESCC. Methods: To identify novel biomarkers for esophageal squamous cell carcinoma (ESCC), specimens from 10 patients with ESCC were subjected to a comparative proteomic analysis. The proteomic patterns of ESCC samples and normal esophageal epithelial tissues (NEETs) were compared using two-dimensional gel electrophoresis. And differentially expressed proteins were identified using MALDI-TOF-MS/MS. For further identification of protein in selected spot, western blotting and immunohistochemistry were employed. Results: Twelve proteins were up-regulated and fifteen proteins were down-regulated in the ESCC samples compared with the NEET samples. Up-regulation of galectin-7 was further confirmed by western blotting and immunohistochemistry. Furthermore, immunohistochemical staining of galectin-7 was performed on a tissue microarray containing ESCC samples (n = 50) and NEET samples (n = 10). The expression levels of galectin-7 were markedly higher in the ESCC samples than in the NEET samples (P = 0.012). In addition, tissue microarray analysis also showed that the expression level of galectin-7 was related to the differentiation of ESCC. Conclusions: The present proteomics analysis revealed that galectin-7 was highly expressed in ESCC tissues. The alteration in the expression of galectin-7 was confirmed using a tissue microarray. These findings suggest that galectin-7 could be used as a potential biomarker for ESCC. |
| 学科主题 | Oncology |
| 类目[WOS] | Oncology |
| 关键词[WOS] | HISTOLOGICAL DIFFERENTIATION ; HYPOPHARYNGEAL CANCER ; P53-INDUCED GENE-1 ; MASS SPECTROMETRY ; DOWN-REGULATION ; ANIMAL LECTINS ; CATHEPSIN-B ; ANNEXIN A1 ; EXPRESSION ; PROTEIN |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000279795900005 |
| 版本 | 出版稿 |
| 源URL | [http://202.127.25.143/handle/331003/1105]  |
| 专题 | 上海生化细胞研究所_上海生科院生化细胞研究所 |
| 推荐引用方式 GB/T 7714 | Zhu, X,Ding, M,Yu, ML,et al. Identification of galectin-7 as a potential biomarker for esophageal squamous cell carcinoma by proteomic analysis[J]. BMC CANCER,2010,10(1):290-290. |
| APA | Zhu, X,Ding, M,Yu, ML,Feng, MX,Tan, LJ,&Zhao, FK.(2010).Identification of galectin-7 as a potential biomarker for esophageal squamous cell carcinoma by proteomic analysis.BMC CANCER,10(1),290-290. |
| MLA | Zhu, X,et al."Identification of galectin-7 as a potential biomarker for esophageal squamous cell carcinoma by proteomic analysis".BMC CANCER 10.1(2010):290-290. |
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