Functional characterization of leucine-specific domain 1 from eukaryal and archaeal leucyl-tRNA synthetases
文献类型：期刊论文
| 作者 | Zhou, XL; Wang, M; Tan, M; Huang, QA; Eriani, G; Wang, ED |
| 刊名 | BIOCHEMICAL JOURNAL
 |
| 出版日期 | 2010 |
| 卷号 | 429期号:1页码:505-513 |
| 关键词 | aminoacylation editing Giardia lamblia leucine-specific domain 1 leucyl-tRNA synthetase Pyrococcus horikoshii |
| 通讯作者 | Wang, ED (reprint author), Chinese Acad Sci, Shanghai Inst Biol Sci, Inst Biochem & Cell Biol, State Key Lab Mol Biol,Grad Sch, 320 Yue Yang Rd, Shanghai 200031, Peoples R China.,edwang@sibs.ac.cn |
| 英文摘要 | LeuRS (leucyl-tRNA synthetase) catalyses the esterification of tRNAs(Leu) with leucine. This family of enzymes is divided into prokaryotic and eukaryal/archaeal groups according to the presence and position of specific insertions and extensions. In the present study, we investigated the function of LSDI (leucine-specific domain I), which is naturally present in eukaryal/archaeal LeuRSs, but absent from prokaryotic LeuRSs. When mutated in their common domain, the eukaryal and archaeal LeuRSs exhibited defects in the first reaction step of amino acid activation with variations of leucine or ATP-binding strength, whereas the tRNA aminoacylation was moderately affected. When the eukaryal extension was mutated, severe tRNA charging defects were observed, suggesting that eukaryotes evolved this LSD1 extension in order to improve the aminoacylation reaction step. The results also showed that the LSD Is from organisms of both groups are dispensable for post-transfer editing. Together, the data provide us with a further understanding of the organization and structure of LeuRS domains. |
| 学科主题 | Biochemistry & Molecular Biology |
| 类目[WOS] | Biochemistry & Molecular Biology |
| 关键词[WOS] | AMINO-ACID ACTIVATION ; AQUIFEX-AEOLICUS ; CRYSTAL-STRUCTURE ; RECOGNITION ; TRNA(LEU) ; COMPLEX ; AMINOACYLATION ; SUBSTRATE ; SELECTION ; BINDING |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000280625400009 |
| 版本 | 出版稿 |
| 源URL | [http://202.127.25.143/handle/331003/1111]  |
| 专题 | 上海生化细胞研究所_上海生科院生化细胞研究所 |
| 推荐引用方式 GB/T 7714 | Zhou, XL,Wang, M,Tan, M,et al. Functional characterization of leucine-specific domain 1 from eukaryal and archaeal leucyl-tRNA synthetases[J]. BIOCHEMICAL JOURNAL,2010,429(1):505-513. |
| APA | Zhou, XL,Wang, M,Tan, M,Huang, QA,Eriani, G,&Wang, ED.(2010).Functional characterization of leucine-specific domain 1 from eukaryal and archaeal leucyl-tRNA synthetases.BIOCHEMICAL JOURNAL,429(1),505-513. |
| MLA | Zhou, XL,et al."Functional characterization of leucine-specific domain 1 from eukaryal and archaeal leucyl-tRNA synthetases".BIOCHEMICAL JOURNAL 429.1(2010):505-513. |
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