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Generation of Pig Induced Pluripotent Stem Cells with a Drug-Inducible System
文献类型：期刊论文
| 作者 | Wu, Z; Chen, JJ; Ren, JT; Bao, L; Liao, J; Cui, C; Rao, LJ; Li, H; Gu, YJ; Dai, HM |
| 刊名 | JOURNAL OF MOLECULAR CELL BIOLOGY
 |
| 出版日期 | 2009 |
| 卷号 | 1期号:1页码:46-54 |
| 关键词 | pluripotency reprogram iPS cells pig ungulate embryonic stem cells |
| 通讯作者 | Xiao, L (reprint author), Chinese Acad Sci, Mol Cell Biol Lab, Inst Biochem & Cell Biol, Cell Bank,Stem Cell Bank,Shanghai Inst Biol Sci, Shanghai 200031, Peoples R China.,leixiao@sibs.ac.cn |
| 英文摘要 | Domesticated ungulate pluripotent embryonic stem (ES) cell lines would be useful for generating precise gene-modified animals. To date, many efforts have been made to establish domesticated ungulate pluripotent ES cells from early embryos without success. Here, we report the generation of porcine-induced pluripotent stem (iPS) cells using drug-inducible expression of defined factors. We showed that porcine iPS cells expressed alkaline phosphatase, SSEA3, SSEA4, Tra-1-60, Tra-1-81, Oct3/4, Nanog, Sox2, Rex1 and CDH1. Pig iPS cells expressed high levels of telomerase activity and showed normal karyotypes. These cells could differentiate into cell types of all three germ layers in vitro and in teratomas. Our study reveals properties of porcine pluripotent stem cells that may facilitate the eventual establishment of porcine ES cells. Moreover, the porcine iPS cells produced may be directly useful for the generation of precise gene-modified pigs. |
| 学科主题 | Cell Biology |
| 类目[WOS] | Cell Biology |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000207813000011 |
| 版本 | 出版稿 |
| 源URL | [http://202.127.25.143/handle/331003/1116]  |
| 专题 | 上海生化细胞研究所_上海生科院生化细胞研究所 |
| 推荐引用方式 GB/T 7714 | Wu, Z,Chen, JJ,Ren, JT,et al. Generation of Pig Induced Pluripotent Stem Cells with a Drug-Inducible System[J]. JOURNAL OF MOLECULAR CELL BIOLOGY,2009,1(1):46-54. |
| APA | Wu, Z.,Chen, JJ.,Ren, JT.,Bao, L.,Liao, J.,...&Xiao, L.(2009).Generation of Pig Induced Pluripotent Stem Cells with a Drug-Inducible System.JOURNAL OF MOLECULAR CELL BIOLOGY,1(1),46-54. |
| MLA | Wu, Z,et al."Generation of Pig Induced Pluripotent Stem Cells with a Drug-Inducible System".JOURNAL OF MOLECULAR CELL BIOLOGY 1.1(2009):46-54. |
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