Dynamic proteomics for investigating the response of individual cancer cells under drug action
文献类型：期刊论文
| 作者 | Li, RX; Zeng, R |
| 刊名 | EXPERT REVIEW OF PROTEOMICS
 |
| 出版日期 | 2009 |
| 卷号 | 6期号:1页码:19-21 |
| 关键词 | cell-cell variability central Dogma-tagging method dynamic proteomics approach translocation |
| 通讯作者 | Zeng, R (reprint author), Chinese Acad Sci, Key Lab Syst Biol, Inst Biochem & Cell Biol, Shanghai Inst Biol Sci, Shanghai 200031, Peoples R China.,rongxiali@sibs.ac.cn ; zr@sibs.ac.cn |
| 英文摘要 | One of the greatest challenges in cancer chemotherapy is that seemingly identical cancer cells can respond differently to drug treatment. The pioneering work reported by Cohen and colleagues moves one step closer to solving this challenge. They develop a dynamic proteomics approach that utilizes fluorescent markers and a time-lapse microscope to detect the fluctuating locations and levels of approximately 1000 proteins in individual cancer cells at high temporal resolution. After adminstration of the cancer drug camptothecin, certain proteins display similar spatiotemporal distribution patterns in individual cells; for example, the drug target topoisomerase-1 shows a rapid decrease in protein level and in nuclear location. However, two particular proteins demonstrate cell-cell variability in their behavior corresponding to cell fate, which may help to explain drug resistance. This method offers an effective way to investigate drug mechanisms in individual cells. |
| 学科主题 | Biochemistry & Molecular Biology |
| 类目[WOS] | Biochemical Research Methods |
| 关键词[WOS] | VIEW |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000263761400004 |
| 版本 | 出版稿 |
| 源URL | [http://202.127.25.143/handle/331003/1121]  |
| 专题 | 上海生化细胞研究所_上海生科院生化细胞研究所 |
| 推荐引用方式 GB/T 7714 | Li, RX,Zeng, R. Dynamic proteomics for investigating the response of individual cancer cells under drug action[J]. EXPERT REVIEW OF PROTEOMICS,2009,6(1):19-21. |
| APA | Li, RX,&Zeng, R.(2009).Dynamic proteomics for investigating the response of individual cancer cells under drug action.EXPERT REVIEW OF PROTEOMICS,6(1),19-21. |
| MLA | Li, RX,et al."Dynamic proteomics for investigating the response of individual cancer cells under drug action".EXPERT REVIEW OF PROTEOMICS 6.1(2009):19-21. |
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