Conserved amino acids W423 and N424 in receptor-binding domain of SARS-CoV are potential targets for therapeutic monoclonal antibody
文献类型：期刊论文
| 作者 | Bian, C; Zhang, XQ; Cai, XF; Zhang, LQ; Chen, ZW; Zha, Y; Xu, Y; Xu, K; Lu, W; Yan, LC |
| 刊名 | VIROLOGY
 |
| 出版日期 | 2009 |
| 卷号 | 383期号:1页码:39-46 |
| 关键词 | SARS-CoV Receptor-binding domain Neutralization Monoclonal antibody Epitope Chimeric |
| 通讯作者 | Sun, B (reprint author), Chinese Acad Sci, Shanghai Inst Biol Sci, Inst Biochem & Cell Biol, Mol Cell Biol Lab, 320 Yueyang Rd, Shanghai 200031, Peoples R China.,bsun@sibs.ac.cn |
| 英文摘要 | The receptor-binding domain (RBD) on spike protein of severe acute respiratory syndrome-associated coronavirus (SARS-CoV) is the main region interacting with the viral receptor-ACE2 and is a useful target for induction of neutralizing antibodies against SARS-CoV infection. Here we generated two monoclonal antibodies (mAbs), targeting RBD, with marked virus neutralizing activity. The mAbs recognize a new conformational epitope which consists of several discontinuous peptides (aa. 343-367, 373-390 and 411-428) and is spatially located neighboring the receptor-binding motif (RPM) region of the RBD. Importantly, W423 and N424 residues are essential for mAb recognition and are highly conserved among 107 different strains of SARS, indicating that the residues are the most critical in the epitope which is a novel potential target for therapeutic mAbs. A human-mouse chimeric antibody, based upon the original murine mAb, was also constructed and shown to possess good neutralizing activity and high affinity. (c) 2008 Elsevier Inc. All rights reserved. |
| 学科主题 | Virology |
| 类目[WOS] | Virology |
| 关键词[WOS] | ACUTE RESPIRATORY SYNDROME ; ANGIOTENSIN-CONVERTING ENZYME-2 ; CORONAVIRUS-S-PROTEIN ; SPIKE PROTEIN ; NEUTRALIZING ANTIBODIES ; VIRUS ; GLYCOPROTEIN ; VACCINE ; ENTRY ; 80R |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000262447300006 |
| 版本 | 出版稿 |
| 源URL | [http://202.127.25.143/handle/331003/1123]  |
| 专题 | 上海生化细胞研究所_上海生科院生化细胞研究所 |
| 推荐引用方式 GB/T 7714 | Bian, C,Zhang, XQ,Cai, XF,et al. Conserved amino acids W423 and N424 in receptor-binding domain of SARS-CoV are potential targets for therapeutic monoclonal antibody[J]. VIROLOGY,2009,383(1):39-46. |
| APA | Bian, C.,Zhang, XQ.,Cai, XF.,Zhang, LQ.,Chen, ZW.,...&Sun, B.(2009).Conserved amino acids W423 and N424 in receptor-binding domain of SARS-CoV are potential targets for therapeutic monoclonal antibody.VIROLOGY,383(1),39-46. |
| MLA | Bian, C,et al."Conserved amino acids W423 and N424 in receptor-binding domain of SARS-CoV are potential targets for therapeutic monoclonal antibody".VIROLOGY 383.1(2009):39-46. |
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