The optional long 5 '-untranslated region of human ACAT1 mRNAs impairs the production of ACAT1 protein by promoting its mRNA decay
文献类型：期刊论文
| 作者 | Zhao, XN; Chen, J; Lei, L; Hu, GJ; Xiong, Y; Xu, JJ; Li, Q; Yang, XY; Chang, CCY; Song, BL |
| 刊名 | ACTA BIOCHIMICA ET BIOPHYSICA SINICA
 |
| 出版日期 | 2009 |
| 卷号 | 41期号:1页码:30-41 |
| 关键词 | human ACAT1 mRNA long 5 '-UTR mRNA stability mRNA decay ACAT1 protein production |
| 通讯作者 | Song, BL (reprint author), Chinese Acad Sci, State Key Lab Mol Biol, Inst Biochem & Cell Biol, Shanghai Inst Biol Sci, Shanghai 200031, Peoples R China.,blli@sibs.ac.cn |
| 英文摘要 | We have previously reported that human ACAT1 mRNAs produce the 50 kDa protein using the AUG(1397-1399) initiation codon, and also a minor 56 kDa isoform using the upstream in-frame GGC(1274-1276) initiation codon. The GGC(1274-1276) codon is located at the optional long 5'-untranslated region (5'-UTR, nt 1-1396) of the mRNAs. The DNA sequences corresponding to this 5'-UTR are located in two different chromosomes, 7 and 1. In the current work, we report that the optional long 5'-UTR significantly impairs the production of human ACAT1 protein initiated from the AUG(1397-1399) codon, mainly by promoting its mRNA decay. The western blot analyses indicated that the optional long 5'-UTR potently impaired the production of different proteins initiated from the AUG(1397-1399) codon, meaning that this impairing effect was not influenced by the 3'-UTR or the coding sequence of ACAT1 mRNA. The results of reverse transcription-quantitative polymerase chain reaction demonstrated that this 5'-UTR dramatically reduced the contents of its linked mRNAs. Analyses of the protein to mRNA ratios showed that this 5'-UTR mainly decreased its mRNA stability rather than altering its translational efficiency. We next performed the plasmid transfection experiments and used actinomycin D to inhibit transcription. The results showed that this 5'-UTR promoted its mRNA decay. Additional transfection and nucleofection experiments using RNAs prepared in vitro illustrated that, in both the cytoplasm and the nucleus of cells, the optional long 5'-UTR-linked mRNAs decayed faster than those without the link. Overall, our study brings new insight to the regulation of the human ACAT1 gene expression at the post-transcription level. |
| 学科主题 | Biochemistry & Molecular Biology; Biophysics |
| 类目[WOS] | Biochemistry & Molecular Biology ; Biophysics |
| 关键词[WOS] | A-CHOLESTEROL ACYLTRANSFERASE ; 2 DIFFERENT CHROMOSOMES ; ACYL-COENZYME ; MAMMALIAN-CELLS ; IN-VITRO ; TRANSLATION INITIATION ; SECONDARY STRUCTURES ; GENE-EXPRESSION ; RICH ELEMENTS ; COA |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000264455000004 |
| 版本 | 出版稿 |
| 源URL | [http://202.127.25.143/handle/331003/1124]  |
| 专题 | 上海生化细胞研究所_上海生科院生化细胞研究所 |
| 推荐引用方式 GB/T 7714 | Zhao, XN,Chen, J,Lei, L,et al. The optional long 5 '-untranslated region of human ACAT1 mRNAs impairs the production of ACAT1 protein by promoting its mRNA decay[J]. ACTA BIOCHIMICA ET BIOPHYSICA SINICA,2009,41(1):30-41. |
| APA | Zhao, XN.,Chen, J.,Lei, L.,Hu, GJ.,Xiong, Y.,...&Li, B.(2009).The optional long 5 '-untranslated region of human ACAT1 mRNAs impairs the production of ACAT1 protein by promoting its mRNA decay.ACTA BIOCHIMICA ET BIOPHYSICA SINICA,41(1),30-41. |
| MLA | Zhao, XN,et al."The optional long 5 '-untranslated region of human ACAT1 mRNAs impairs the production of ACAT1 protein by promoting its mRNA decay".ACTA BIOCHIMICA ET BIOPHYSICA SINICA 41.1(2009):30-41. |
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