Inhibition of renal cancer cell growth in vitro and in vivo with oncolytic adenovirus armed short hairpin RNA targeting Ki-67 encoding mRNA
文献类型：期刊论文
| 作者 | Zheng, JN; Pei, DS; Mao, LJ; Liu, XY; Mei, DD; Zhang, BF; Shi, Z; Wen, RM; Sun, XQ |
| 刊名 | CANCER GENE THERAPY
 |
| 出版日期 | 2009 |
| 卷号 | 16期号:1页码:20-32 |
| 通讯作者 | Zheng, JN (reprint author), Xuzhou Med Coll, Affiliated Hosp, Lab Urol, 99 W Huai Hai Rd, Xuzhou 221002, Jiangsu, Peoples R China.,jnzheng@xzmc.edu.cn |
| 英文摘要 | RNA interference (RNAi) has been proved to be a powerful tool for gene knockdown purpose and holds great promise for the treatment of cancer. Our previous study demonstrated that the reduction of Ki-67 expression by means of chemically synthesized siRNAs and shRNAs expressed from plasmid resulted in proliferation inhibition in human renal carcinoma cells. In this study, we constructed a novel oncolytic adenovirus-based shRNA expression system, ZD55-Ki67, and explored ZD55-Ki67-mediated RNAi for Ki-67 gene silencing. Our results showed that ZD55-Ki67 could induce silencing of the Ki- 67 gene effectively, allow for efficient tumor-specific viral replication and induce the apoptosis of tumor cells effectively in vitro and in nude mice. We conclude that combining shRNA gene therapy and oncolytic virotherapy can enhance antitumor efficacy as a result of synergism between CRAd oncolysis and shRNA antitumor responses. |
| 学科主题 | Biotechnology & Applied Microbiology; Oncology; Genetics & Heredity; Research & Experimental Medicine |
| 类目[WOS] | Biotechnology & Applied Microbiology ; Oncology ; Genetics & Heredity ; Medicine, Research & Experimental |
| 关键词[WOS] | SMALL-INTERFERING-RNA ; CONDITIONALLY REPLICATING ADENOVIRUSES ; PHASE-II TRIAL ; GENE-VIROTHERAPY ; NECK-CANCER ; SELECTIVE ADENOVIRUS ; ANTITUMOR-ACTIVITY ; CARCINOMA-CELLS ; E1B-55KD GENE ; EXPRESSION |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000261703100003 |
| 版本 | 出版稿 |
| 源URL | [http://202.127.25.143/handle/331003/1128]  |
| 专题 | 上海生化细胞研究所_上海生科院生化细胞研究所 |
| 推荐引用方式 GB/T 7714 | Zheng, JN,Pei, DS,Mao, LJ,et al. Inhibition of renal cancer cell growth in vitro and in vivo with oncolytic adenovirus armed short hairpin RNA targeting Ki-67 encoding mRNA[J]. CANCER GENE THERAPY,2009,16(1):20-32. |
| APA | Zheng, JN.,Pei, DS.,Mao, LJ.,Liu, XY.,Mei, DD.,...&Sun, XQ.(2009).Inhibition of renal cancer cell growth in vitro and in vivo with oncolytic adenovirus armed short hairpin RNA targeting Ki-67 encoding mRNA.CANCER GENE THERAPY,16(1),20-32. |
| MLA | Zheng, JN,et al."Inhibition of renal cancer cell growth in vitro and in vivo with oncolytic adenovirus armed short hairpin RNA targeting Ki-67 encoding mRNA".CANCER GENE THERAPY 16.1(2009):20-32. |
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