Neuregulin-1 enhances differentiation of cardiomyocytes from embryonic stem cells
文献类型：期刊论文
| 作者 | Wang, Z; Xu, GT; Wu, YL; Guan, Y; Cui, L; Lei, X; Zhang, JF; Mou, LS; Sun, BG; Dai, QY |
| 刊名 | MEDICAL & BIOLOGICAL ENGINEERING & COMPUTING
 |
| 出版日期 | 2009 |
| 卷号 | 47期号:1页码:41-48 |
| 关键词 | Neuregulin-1 Embryonic stem cells Cardiomyocytes Phosphatidylinositol 3-kinase |
| 通讯作者 | Dai, QY (reprint author), Shanghai Jiao Tong Univ, Sch Med, Peoples Hosp 1, Dept Cardiol, 100 Haining Rd, Shanghai 200080, Peoples R China.,qiuyand@yahoo.com |
| 英文摘要 | Neuregulin-1 (NRG-1) is a multifunctional regulator that acts through receptor tyrosine kinases of the epidermal growth factor (EGF/ErbB) receptor family in diverse tissue. ErbB receptors are expressed in developing embryoid bodies (EBs), and the importance of the NRG-1/ErbB signaling axis in heart development has been investigated, but the underlying molecular mechanism is poorly studied. NRG-1 treatment at 100 ng/ml significantly increased the number of beating EBs of differentiated murine embryonic stem cells (ESCs). Furthermore, NRG-1 up-regulated the expression of the cardiac-restricted transcription factors Nkx2.5 and GATA-4 and factors involved in differentiated cardiac cells (alpha-MHC, beta-MHC and alpha-actinin); NRG-1-induced increase of Nkx2.5 transcription was inhibited by treatment with the PI3 K inhibitor or ErbB receptor inhibitor. Western blot analysis confirmed that the expression of phospho-Akt in the beating foci was increased in the presence of NRG-1. Our results suggest that NRG-1 promotes cardiomyocyte differentiation of ESCs and the ErbB/PI3 K/Akt signaling pathway is one of the underlying molecular mechanisms. |
| 学科主题 | Computer Science; Engineering; Mathematical & Computational Biology; Medical Informatics |
| 类目[WOS] | Computer Science, Interdisciplinary Applications ; Engineering, Biomedical ; Mathematical & Computational Biology ; Medical Informatics |
| 关键词[WOS] | PHOSPHATIDYLINOSITOL 3-KINASE ; CARDIAC MYOCYTES ; TRANSCRIPTIONAL ACTIVITY ; IN-VITRO ; ES CELLS ; KINASE-B ; P300 ; GROWTH ; HEART ; GATA-4 |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000262483600006 |
| 版本 | 出版稿 |
| 源URL | [http://202.127.25.143/handle/331003/1136]  |
| 专题 | 上海生化细胞研究所_上海生科院生化细胞研究所 |
| 推荐引用方式 GB/T 7714 | Wang, Z,Xu, GT,Wu, YL,et al. Neuregulin-1 enhances differentiation of cardiomyocytes from embryonic stem cells[J]. MEDICAL & BIOLOGICAL ENGINEERING & COMPUTING,2009,47(1):41-48. |
| APA | Wang, Z.,Xu, GT.,Wu, YL.,Guan, Y.,Cui, L.,...&Dai, QY.(2009).Neuregulin-1 enhances differentiation of cardiomyocytes from embryonic stem cells.MEDICAL & BIOLOGICAL ENGINEERING & COMPUTING,47(1),41-48. |
| MLA | Wang, Z,et al."Neuregulin-1 enhances differentiation of cardiomyocytes from embryonic stem cells".MEDICAL & BIOLOGICAL ENGINEERING & COMPUTING 47.1(2009):41-48. |
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