Transcription level of messenger RNA per gene copy determined with dual-spike-in strategy
文献类型：期刊论文
| 作者 | Zhang, Y; Wei, ZG; Li, YY; Chen, YH; Shen, WD; Lu, CD |
| 刊名 | ANALYTICAL BIOCHEMISTRY
 |
| 出版日期 | 2009 |
| 卷号 | 394期号:2页码:202-208 |
| 关键词 | Quantified transcription level Exogenous RNA and DNA references Dual-spike-in strategy Tissue-specific expression Silkworm Bombyx mori |
| 通讯作者 | Lu, CD (reprint author), Chinese Acad Sci, Shanghai Inst Biol Sci, Inst Biochem & Cell Biol, State Key Lab Mol Biol, Shanghai 200031, Peoples R China.,cdlu@sibs.ac.cn |
| 英文摘要 | To quantify the transcription level of a gene, we have conceived a novel concept, transcription level of messenger RNA (mRNA) per gene copy, which was determined with a dual-spike-in strategy. In this strategy, an exogenous DNA was added as the spike reference for target DNA in addition to the exogenous RNA as the reference for target RNA. After the mRNA-to-DNA ratio of a target gene was estimated by real-time polymerase chain reaction (PCR), it was first normalized with the mRNA-to-DNA ratio of the exogenous reference. The normalized ratio was multiplied by the ratio of exogenous RNA to exogenous DNA to obtain the transcription level of mRNA per gene copy. This quantified transcription value allows one to compare the expression of a target gene in different tissues or the expression in a specified tissue under different conditions. (C) 2009 Elsevier Inc. All rights reserved. |
| 学科主题 | Biochemistry & Molecular Biology; Chemistry |
| 类目[WOS] | Biochemical Research Methods ; Biochemistry & Molecular Biology ; Chemistry, Analytical |
| 关键词[WOS] | TIME QUANTITATIVE PCR ; POLYMERASE CHAIN-REACTION ; BOMBYX-MORI ; RT-PCR ; MICROARRAY ANALYSIS ; NORMALIZATION ; AMPLIFICATION ; EXPRESSION ; MUTAGENESIS ; TISSUES |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000272884000008 |
| 版本 | 出版稿 |
| 源URL | [http://202.127.25.143/handle/331003/1139]  |
| 专题 | 上海生化细胞研究所_上海生科院生化细胞研究所 |
| 推荐引用方式 GB/T 7714 | Zhang, Y,Wei, ZG,Li, YY,et al. Transcription level of messenger RNA per gene copy determined with dual-spike-in strategy[J]. ANALYTICAL BIOCHEMISTRY,2009,394(2):202-208. |
| APA | Zhang, Y,Wei, ZG,Li, YY,Chen, YH,Shen, WD,&Lu, CD.(2009).Transcription level of messenger RNA per gene copy determined with dual-spike-in strategy.ANALYTICAL BIOCHEMISTRY,394(2),202-208. |
| MLA | Zhang, Y,et al."Transcription level of messenger RNA per gene copy determined with dual-spike-in strategy".ANALYTICAL BIOCHEMISTRY 394.2(2009):202-208. |
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