Adipogenesis licensing and execution are disparately linked to cell proliferation
文献类型：期刊论文
| 作者 | Guo, W; Zhang, KM; Tu, K; Li, YX; Zhu, L; Xiao, HS; Yang, Y; Wu, JR |
| 刊名 | CELL RESEARCH
 |
| 出版日期 | 2009 |
| 卷号 | 19期号:2页码:216-223 |
| 关键词 | adipogenesis proliferation contact inhibition DNA methylation C/EBP alpha |
| 通讯作者 | Wu, JR (reprint author), Chinese Acad Sci, Shanghai Inst Biol Sci, Inst Biochem & Cell Biol, Key Lab Syst Biol, 320 Yue Yang Rd, Shanghai 200031, Peoples R China.,wujr@sibs.ac.cn |
| 英文摘要 | Coordination of cell differentiation and proliferation is a key issue in the development process of multi-cellular organisms and stem cells. Here we provide evidence that the establishment of adipocyte differentiation of 3T3-L1 cells requires two processes: the licensing of an adipogenesis gene-expression program within a particular growth-arrest stage, i.e., the contact-inhibition stage, and then the execution of this program in a cell-cycle-independent manner, by which the licensed progenitors are differentiated into adipocytes in the presence of inducing factors. Our results showed that differentiation licensing of 3T3-L1 cells during the contact-inhibition stage involved epigenetic modifications such as DNA methylation and histone modifications, whereas disturbing these epigenetic modifications by DNA methylation inhibitors or RNAi during the contact-inhibition stage significantly reduced adipogenesis efficiency. More importantly, when these licensed 3T3-L1 cells were re-cultured under non-differentiating conditions or treated only with insulin, this adipogenesis commitment could be maintained from one cell generation to the next, whereby the licensed program could be activated in a cell-cycle-independent manner once these cells were subjected to adipogenesis-inducing conditions. This result suggests that differentiation licensing and differentiation execution can be uncoupled and disparately linked to cell proliferation. Our findings deliver a new concept that cell-fate decision can be subdivided into at least two stages, licensing and execution, which might have different regulatory relationships with cell proliferation. In addition, this new concept may provide a clue for developing new strategies against obesity. |
| 学科主题 | Cell Biology |
| 类目[WOS] | Cell Biology |
| 关键词[WOS] | MITOTIC CLONAL EXPANSION ; 3T3-L1 PREADIPOCYTE DIFFERENTIATION ; ADIPOCYTE DIFFERENTIATION ; LEUKEMIC-CELLS ; GROWTH ARREST ; STEM-CELL ; RECEPTOR ; CYCLE ; INHIBITION ; PHASE |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000263287500008 |
| 版本 | 出版稿 |
| 源URL | [http://202.127.25.143/handle/331003/1150]  |
| 专题 | 上海生化细胞研究所_上海生科院生化细胞研究所 |
| 推荐引用方式 GB/T 7714 | Guo, W,Zhang, KM,Tu, K,et al. Adipogenesis licensing and execution are disparately linked to cell proliferation[J]. CELL RESEARCH,2009,19(2):216-223. |
| APA | Guo, W.,Zhang, KM.,Tu, K.,Li, YX.,Zhu, L.,...&Wu, JR.(2009).Adipogenesis licensing and execution are disparately linked to cell proliferation.CELL RESEARCH,19(2),216-223. |
| MLA | Guo, W,et al."Adipogenesis licensing and execution are disparately linked to cell proliferation".CELL RESEARCH 19.2(2009):216-223. |
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