Functional characterization of the human-specific (type II) form of kallikrein 8, a gene involved in learning and memory
文献类型：期刊论文
| 作者 | Lu, ZX; Huang, Q; Su, B |
| 刊名 | CELL RESEARCH
 |
| 出版日期 | 2009 |
| 卷号 | 19期号:2页码:259-267 |
| 关键词 | type II KLK8 alternative splicing cognition human evolution |
| 通讯作者 | Su, B (reprint author), Chinese Acad Sci, Kunming Inst Zool, State Key Lab Genet Resources & Evolut, Kunming 650223, Peoples R China.,sub@mail.kiz.ac.cn |
| 英文摘要 | Kallikrein 8 (KLK8) is a serine protease functioning in the central nervous system, and essential in many aspects of neuronal activities. Sequence comparison and gene expression analysis among diverse primate species identified a human-specific splice form of KLK8 (type II) with preferential expression in the human brain, which may contribute to the origin of human cognition. To gain insights into the physiological and biochemical role of this novel form, we conducted functional analyses of human type II KLK8. Our results show that type II KLK8 is abundantly expressed in human embryonic stem cells and in embryo brain samples, suggesting a potential role in embryogenesis. There are dramatic expression variations in different individuals and brain regions, which is a reflection of its dynamic role in neural activities. Furthermore, the transcription start site (TSS) of KLK8 is tissue-specific, with a brain-specific TSS found in humans indicating functional specialization. Our in vitro biochemical assay shows that there is a type II-specific intermediate protein form, although the processed end-point enzymes are the same for both type I and type II KLK8, suggesting that the emergence of type II KLK8 in the human brain likely leads to functional modifications of KLK8. |
| 学科主题 | Cell Biology |
| 类目[WOS] | Cell Biology |
| 关键词[WOS] | NEUROPSIN MESSENGER-RNA ; HUMAN TISSUE KALLIKREINS ; LONG-TERM POTENTIATION ; SERINE-PROTEASE ; UNTRANSLATED REGIONS ; MOUSE-BRAIN ; IN-VIVO ; EXPRESSION ; HIPPOCAMPUS ; NEURONS |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000263287500012 |
| 版本 | 出版稿 |
| 源URL | [http://202.127.25.143/handle/331003/1151]  |
| 专题 | 上海生化细胞研究所_上海生科院生化细胞研究所 |
| 推荐引用方式 GB/T 7714 | Lu, ZX,Huang, Q,Su, B. Functional characterization of the human-specific (type II) form of kallikrein 8, a gene involved in learning and memory[J]. CELL RESEARCH,2009,19(2):259-267. |
| APA | Lu, ZX,Huang, Q,&Su, B.(2009).Functional characterization of the human-specific (type II) form of kallikrein 8, a gene involved in learning and memory.CELL RESEARCH,19(2),259-267. |
| MLA | Lu, ZX,et al."Functional characterization of the human-specific (type II) form of kallikrein 8, a gene involved in learning and memory".CELL RESEARCH 19.2(2009):259-267. |
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