Interactions of Na+,K+-ATPase and co-expressed delta-opioid receptor
文献类型：期刊论文
| 作者 | Deng, HP; Yang, ZJ; Li, YT; Bao, GB; Friedrich, T; Gu, QB; Shen, XY; Schwarz, W |
| 刊名 | NEUROSCIENCE RESEARCH
 |
| 出版日期 | 2009 |
| 卷号 | 65期号:3页码:222-227 |
| 关键词 | Xenopus oocytes Hippocampal neurons Na+ delta-Opioid receptor Protein-protein interaction Pain suppression K+-ATPase |
| 通讯作者 | Schwarz, W (reprint author), Max Planck Inst Biophys, Max von Laue Str 3, D-60438 Frankfurt, Germany., |
| 英文摘要 | To investigate interference of delta-opioid receptor with the Na+,K+-ATPase in a simple model system, we used the Xenopus oocytes as an expression system. Our results indicate that expression of the delta-opioid receptor (DOR) results in reduction of endogenous sodium-pump activity. Stimulation of DOR by the DOR agonist [(D)-Pen(2,5)]-enkephalin (DPDPE) had no pronounced additional effect on pump activity. Qualitatively similar results were obtained in experiments with a variety of co-expressed exogenous sodium Pumps. We suggest that reduced pump activity with DOR expression is brought about by an interaction of the pump with DOR. Direct interaction is also supported by co-immunoprecipitation, not only in the Xenopus oocytes but also in rat hippocampal neurons The interaction may be responsible for altered agonist sensitivity of DOR: activation of the sodium pump led to an increase of the K-m value for DOR activation by DPDPE from about 0.17 to 0.27 mu M. In conclusion, pump activity not only affects neural activity directly but our results also suggest that pump activity is affected through functional interaction with DOR that will modulate pain sensation. (C) 2009 Elsevier Ireland Ltd and the Japan Neuroscience Society. All rights reserved. |
| 学科主题 | Neurosciences & Neurology |
| 类目[WOS] | Neurosciences |
| 关键词[WOS] | GLUTAMATE TRANSPORTER EAAC1 ; XENOPUS-LAEVIS OOCYTES ; SODIUM-PUMP ACTIVITY ; NA+/K+-ATPASE ; K+-ATPASE ; ACTIVATION ; EXPRESSION ; MORPHINE ; RAT ; INVOLVEMENT |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000271131100002 |
| 版本 | 出版稿 |
| 源URL | [http://202.127.25.143/handle/331003/1156]  |
| 专题 | 上海生化细胞研究所_上海生科院生化细胞研究所 |
| 推荐引用方式 GB/T 7714 | Deng, HP,Yang, ZJ,Li, YT,et al. Interactions of Na+,K+-ATPase and co-expressed delta-opioid receptor[J]. NEUROSCIENCE RESEARCH,2009,65(3):222-227. |
| APA | Deng, HP.,Yang, ZJ.,Li, YT.,Bao, GB.,Friedrich, T.,...&Schwarz, W.(2009).Interactions of Na+,K+-ATPase and co-expressed delta-opioid receptor.NEUROSCIENCE RESEARCH,65(3),222-227. |
| MLA | Deng, HP,et al."Interactions of Na+,K+-ATPase and co-expressed delta-opioid receptor".NEUROSCIENCE RESEARCH 65.3(2009):222-227. |
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