An essential role for DNA methyltransferase 3a in melanoma tumorigenesis
文献类型：期刊论文
| 作者 | Deng, T; Kuang, Y; Wang, L; Li, J; Wang, ZG; Fei, J |
| 刊名 | BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
 |
| 出版日期 | 2009 |
| 卷号 | 387期号:3页码:611-616 |
| 关键词 | Melanoma DNA methylation Dnmt3a Tumorigenesis Metastasis Immune escape |
| 通讯作者 | Kuang, Y (reprint author), Shanghai Nan Fang Model Organism Res Ctr, Shanghai, Peoples R China.,kuangying01@yahoo.com.cn |
| 英文摘要 | Abnormal DNA methylation and associated silencing Of tumor suppressor genes are common to many types of cancers. Among the three coordinate DNA methyltransferases (Din-tits), Dnmt1 and Dnmt3b were both shown to be important for cancer cell survival and tumorigenesis. However, the relationship between Dnmt3a and tumorigenesis is still largely Unknown. Here, we show that inhibition of Dnmt3a expression, by stable transfection of a Dnmt3a-RNA interference (RNAi) construct dramatically inhibited melanoma growth and metastasis in mouse melanoma models. Microarray analysis revealed that genes critical for the tumor immune response, were implicated in the inhibition of melanoma growth. Expression of a cluster of class I and class II MHC genes, class II transactivator (Ciita), as well as a subset of 5 chemokines (Cxcl9, Cxcl16, Ccl12, Ccl4, and Ccl2) were up-regulated. Furthermore, we determined that the promoter IV of Ciita was significantly demethylated in Dnmt3a-depleted tumors. In addition, several known tumor-related genes, which are critical for developmental processes and cell cycle, were confirmed to be misregulated, including TgfB1, Socs1, Socs2, E2F6, Cene1, and Cyr61. The results presented in this report strongly suggest that Dnmt3a plays all essential role in melanoma tumorigenesis, and that the Underlying mechanisms include the modulation of the tumor immune response, as well as Other processes. (C) 2009 Elsevier Inc. All rights reserved. |
| 学科主题 | Biochemistry & Molecular Biology ; Biophysics |
| 类目[WOS] | Biochemistry & Molecular Biology ; Biophysics |
| 关键词[WOS] | CLASS-I ANTIGEN ; MALIGNANT-MELANOMA ; PROMOTER HYPERMETHYLATION ; EPIGENETIC INACTIVATION ; CUTANEOUS MELANOMA ; TUMOR-GROWTH ; E2F FAMILY ; CYCLIN-E ; CANCER ; METHYLATION |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000269137800037 |
| 版本 | 出版稿 |
| 源URL | [http://202.127.25.143/handle/331003/1159]  |
| 专题 | 上海生化细胞研究所_上海生科院生化细胞研究所 |
| 推荐引用方式 GB/T 7714 | Deng, T,Kuang, Y,Wang, L,et al. An essential role for DNA methyltransferase 3a in melanoma tumorigenesis[J]. BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS,2009,387(3):611-616. |
| APA | Deng, T,Kuang, Y,Wang, L,Li, J,Wang, ZG,&Fei, J.(2009).An essential role for DNA methyltransferase 3a in melanoma tumorigenesis.BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS,387(3),611-616. |
| MLA | Deng, T,et al."An essential role for DNA methyltransferase 3a in melanoma tumorigenesis".BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS 387.3(2009):611-616. |
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