Two tyrosine residues outside the editing active site in Giardia lamblia leucyl-tRNA synthetase are essential for the post-transfer editing
文献类型：期刊论文
| 作者 | Zhou, XL; Wang, ED |
| 刊名 | BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
 |
| 出版日期 | 2009 |
| 卷号 | 386期号:3页码:510-515 |
| 关键词 | CP1 domain Giardia lamblia Leucyl-tRNA synthetase Post-transfer editing Tyrosine |
| 通讯作者 | Wang, ED (reprint author), Chinese Acad Sci, State Key Lab Mol Biol, 320 Yue Yang Rd, Shanghai 200031, Peoples R China.,edwang@sibs.ac.cn |
| 英文摘要 | Leucyl-tRNA synthetase (LeuRS) is responsible for the LeU-tRNA(Leu) synthesis. The connective peptide 1 (CP1) domain inserted into the Rossmann nuclecitide binding fold possesses editing active site to hydrolyze the mischarged tRNA(Leu) with noncognate amino acid, then to ensure high fidelity of protein synthesis. A few co-crystal structures of LeuRS with tRNA(Leu) in different conformations revealed that tRNA(Leu) 3' end shuttled between synthetic and editing active sites dynamically with direct and specific interaction with the CP1 domain. Here, we reported that Y515 and Y520 outside the editing active site of CP1 domain of Giardia lamblia LeuRS (G/LeuRS) are crucial for post-transfer editing by influencing the binding affinity with mischarged tRNA. Mutations on Y515 and Y520 also decreased tRNA(Leu) charging activity to various extents but had no effect on leucine activation. Our results gave some biochemical knowledge about interaction of tRNA(Leu) 3' end with the CP1 domain in archaeal/eukaryotic LeuRS. (C) 2009 Elsevier Inc. All rights reserved. |
| 学科主题 | Biochemistry & Molecular Biology; Biophysics |
| 类目[WOS] | Biochemistry & Molecular Biology ; Biophysics |
| 关键词[WOS] | CRYSTAL-STRUCTURE ; TRNA(LEU) ; COMPLEX ; DOMAIN ; DISCRIMINATION ; EVOLUTION |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000268262100019 |
| 版本 | 出版稿 |
| 源URL | [http://202.127.25.143/handle/331003/1160]  |
| 专题 | 上海生化细胞研究所_上海生科院生化细胞研究所 |
| 推荐引用方式 GB/T 7714 | Zhou, XL,Wang, ED. Two tyrosine residues outside the editing active site in Giardia lamblia leucyl-tRNA synthetase are essential for the post-transfer editing[J]. BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS,2009,386(3):510-515. |
| APA | Zhou, XL,&Wang, ED.(2009).Two tyrosine residues outside the editing active site in Giardia lamblia leucyl-tRNA synthetase are essential for the post-transfer editing.BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS,386(3),510-515. |
| MLA | Zhou, XL,et al."Two tyrosine residues outside the editing active site in Giardia lamblia leucyl-tRNA synthetase are essential for the post-transfer editing".BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS 386.3(2009):510-515. |
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