MicroRNA detection by microarray
文献类型：期刊论文
| 作者 | Li, W; Ruan, KC |
| 刊名 | ANALYTICAL AND BIOANALYTICAL CHEMISTRY
 |
| 出版日期 | 2009 |
| 卷号 | 394期号:4页码:1117-1124 |
| 关键词 | MicroRNA MicroRNA detection MicroRNA microarray MicroRNA labeling |
| 通讯作者 | Ruan, KC (reprint author), Chinese Acad Sci, Shanghai Inst Biol Sci, Inst Biochem & Cell Biol, State Key Lab Mol Biol, 320 Yue Yang Rd, Shanghai 200031, Peoples R China.,kcruan@sibs.ac.cn |
| 英文摘要 | MicroRNAs (miRNAs) are a class of small noncoding RNAs similar to 22 nt in length that regulate gene expression and play fundamental roles in multiple biological processes, including cell differentiation, proliferation and apoptosis as well as disease processes. The study of miRNA has thus become a rapidly emerging field in life science. The detection of miRNA expression is a very important first step in miRNA exploration. Several methodologies, including cloning, northern blotting, real-time RT-PCR, microRNA arrays and ISH (in situ hybridization), have been developed and applied successfully in miRNA profiling. This review discusses the main existing microRNA detection technologies, while emphasizing microRNA arrays. |
| 学科主题 | Biochemistry & Molecular Biology; Chemistry |
| 类目[WOS] | Biochemical Research Methods ; Chemistry, Analytical |
| 关键词[WOS] | CAENORHABDITIS-ELEGANS ; GENE-EXPRESSION ; MAMMALIAN MICRORNAS ; NORTHERN BLOT ; SMALL RNAS ; RT-PCR ; REVEALS ; IDENTIFICATION ; TISSUES ; MIRNAS |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000266160400023 |
| 版本 | 出版稿 |
| 源URL | [http://202.127.25.143/handle/331003/1173]  |
| 专题 | 上海生化细胞研究所_上海生科院生化细胞研究所 |
| 推荐引用方式 GB/T 7714 | Li, W,Ruan, KC. MicroRNA detection by microarray[J]. ANALYTICAL AND BIOANALYTICAL CHEMISTRY,2009,394(4):1117-1124. |
| APA | Li, W,&Ruan, KC.(2009).MicroRNA detection by microarray.ANALYTICAL AND BIOANALYTICAL CHEMISTRY,394(4),1117-1124. |
| MLA | Li, W,et al."MicroRNA detection by microarray".ANALYTICAL AND BIOANALYTICAL CHEMISTRY 394.4(2009):1117-1124. |
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