Potent antitumor activity of double-regulated oncolytic adenovirus-mediated ST13 for colorectal cancer
文献类型：期刊论文
| 作者 | Yu, DB; Zhong, SY; Yang, M; Wang, YG; Qian, QJ; Zheng, S; Liu, XY |
| 刊名 | CANCER SCIENCE
 |
| 出版日期 | 2009 |
| 卷号 | 100期号:4页码:678-683 |
| 通讯作者 | Liu, XY (reprint author), Zhejiang Sci Tech Univ, Xinyuan Inst Med & Biotechnol, Hangzhou, Zhejiang, Peoples R China.,zhengshu@zju.edu.cn ; xyliu@sibs.ac.cn |
| 英文摘要 | Following targeted gene virotherapy, the suppression of tumorigenicity 13 (ST13) gene was inserted into the double-regulated oncolytic adenovirus SG500 to ensure more safety and potent antitumor activity against colorectal cancer in vitro and in vivo. We generated the ST13-expressing oncolytic adenovirus SG500-ST13, with which colorectal carcinoma cell lines SW620 and HCT116, and the lung fibroblast cell line WI38, were infected. Crystal violet staining was carried out to detect the cytopathic effect in cells, and the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide method was used to assay cell viability. The effect of apoptosis induced by SG500-ST13 was confirmed by Hoechst staining and the TdT-mediated dUTP-biotin nick-end labeling method. To further identify the antitumor effects of SG500-ST13 on HCT116 xenografts in Balb/c nude mice, the induction of cell death was assessed by hematoxylin-eosin staining. Immunohistochemical study was also carried out. (Cancer Sci 2009; 100: 678-683) |
| 学科主题 | Oncology |
| 类目[WOS] | Oncology |
| 关键词[WOS] | TARGETING HIF-1 ; PROTEIN ; HIP ; CHAPERONE ; PROMOTER ; EFFICACY ; THERAPY ; AGENTS ; DEATH |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000265578100017 |
| 版本 | 出版稿 |
| 源URL | [http://202.127.25.143/handle/331003/1177]  |
| 专题 | 上海生化细胞研究所_上海生科院生化细胞研究所 |
| 推荐引用方式 GB/T 7714 | Yu, DB,Zhong, SY,Yang, M,et al. Potent antitumor activity of double-regulated oncolytic adenovirus-mediated ST13 for colorectal cancer[J]. CANCER SCIENCE,2009,100(4):678-683. |
| APA | Yu, DB.,Zhong, SY.,Yang, M.,Wang, YG.,Qian, QJ.,...&Liu, XY.(2009).Potent antitumor activity of double-regulated oncolytic adenovirus-mediated ST13 for colorectal cancer.CANCER SCIENCE,100(4),678-683. |
| MLA | Yu, DB,et al."Potent antitumor activity of double-regulated oncolytic adenovirus-mediated ST13 for colorectal cancer".CANCER SCIENCE 100.4(2009):678-683. |
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