中国科学院机构知识库网格
Chinese Academy of Sciences Institutional Repositories Grid
Potent antitumor activity of double-regulated oncolytic adenovirus-mediated ST13 for colorectal cancer

文献类型:期刊论文

作者Yu, DB; Zhong, SY; Yang, M; Wang, YG; Qian, QJ; Zheng, S; Liu, XY
刊名CANCER SCIENCE
出版日期2009
卷号100期号:4页码:678-683
通讯作者Liu, XY (reprint author), Zhejiang Sci Tech Univ, Xinyuan Inst Med & Biotechnol, Hangzhou, Zhejiang, Peoples R China.,zhengshu@zju.edu.cn ; xyliu@sibs.ac.cn
英文摘要Following targeted gene virotherapy, the suppression of tumorigenicity 13 (ST13) gene was inserted into the double-regulated oncolytic adenovirus SG500 to ensure more safety and potent antitumor activity against colorectal cancer in vitro and in vivo. We generated the ST13-expressing oncolytic adenovirus SG500-ST13, with which colorectal carcinoma cell lines SW620 and HCT116, and the lung fibroblast cell line WI38, were infected. Crystal violet staining was carried out to detect the cytopathic effect in cells, and the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide method was used to assay cell viability. The effect of apoptosis induced by SG500-ST13 was confirmed by Hoechst staining and the TdT-mediated dUTP-biotin nick-end labeling method. To further identify the antitumor effects of SG500-ST13 on HCT116 xenografts in Balb/c nude mice, the induction of cell death was assessed by hematoxylin-eosin staining. Immunohistochemical study was also carried out. (Cancer Sci 2009; 100: 678-683)
学科主题Oncology
类目[WOS]Oncology
关键词[WOS]TARGETING HIF-1 ; PROTEIN ; HIP ; CHAPERONE ; PROMOTER ; EFFICACY ; THERAPY ; AGENTS ; DEATH
收录类别SCI
语种英语
WOS记录号WOS:000265578100017
版本出版稿
源URL[http://202.127.25.143/handle/331003/1177]  
专题上海生化细胞研究所_上海生科院生化细胞研究所
推荐引用方式
GB/T 7714
Yu, DB,Zhong, SY,Yang, M,et al. Potent antitumor activity of double-regulated oncolytic adenovirus-mediated ST13 for colorectal cancer[J]. CANCER SCIENCE,2009,100(4):678-683.
APA Yu, DB.,Zhong, SY.,Yang, M.,Wang, YG.,Qian, QJ.,...&Liu, XY.(2009).Potent antitumor activity of double-regulated oncolytic adenovirus-mediated ST13 for colorectal cancer.CANCER SCIENCE,100(4),678-683.
MLA Yu, DB,et al."Potent antitumor activity of double-regulated oncolytic adenovirus-mediated ST13 for colorectal cancer".CANCER SCIENCE 100.4(2009):678-683.

入库方式: OAI收割

来源:上海生物化学与细胞生物学研究所

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