The differential protein and lipid compositions of noncaveolar lipid microdomains and caveolae
文献类型：期刊论文
| 作者 | Yao, Y; Hong, SY; Zhou, H; Yuan, TC; Zeng, R; Liao, K |
| 刊名 | CELL RESEARCH
 |
| 出版日期 | 2009 |
| 卷号 | 19期号:4页码:497-506 |
| 关键词 | membrane lipid microdomains caveolae noncaveolar lipid microdomains lipid rafts sucrose density gradient sodium carbonate extraction |
| 通讯作者 | Liao, K (reprint author), Chinese Acad Sci, Shanghai Inst Biol Sci, Inst Biochem & Cell Biol, State Key Lab Mol Biol, Shanghai 200031, Peoples R China.,kliao@sibs.ac.cn |
| 英文摘要 | Morphologically, caveolae and lipid rafts are two different membrane structures. They are often reported to share similar lipid and protein compositions, and are considered to be two subtypes of membrane lipid microdomains. By modifying sucrose density gradient flotation centrifugation, which is used to isolate lipid microdomains, we were able to separate caveolae and noncaveolar lipid microdomains into two distinct fractions. The caveolar membranes are membrane vesicles of 100-nm diameter, enriched with caveolin-1 and flotillin-1. The noncaveolar lipid microdomains are amorphous membranes and most likely the coalescence of heterogeneous lipid rafts. They are depleted of caveolin-1 and are more enriched with cholesterol and sphingolipids than the caveolae. Many membrane proteins, such as insulin-like growth factor-1 receptor (membrane receptor), aquaporin-1 (membrane transporter), Thy-1 and N-cadherin (glycosylphosphatidylinositol-anchored membrane protein and membrane glycoprotein), are specifically associated with noncaveolar lipid microdomains, but not with caveolae. These results indicate that the lipid and protein compositions of caveolae differ from those of noncaveolar lipid microdomains. The difference in their protein compositions implies that these two membrane microdomains may have different cellular functions. |
| 学科主题 | Cell Biology |
| 类目[WOS] | Cell Biology |
| 关键词[WOS] | SIGNAL-TRANSDUCING MOLECULES ; INSULIN-RECEPTOR SUBSTRATE-1 ; THIN-LAYER CHROMATOGRAPHY ; CHOLESTEROL DEPLETION ; PLASMA-MEMBRANE ; CELL-MEMBRANES ; T-CADHERIN ; RAFTS ; DOMAINS ; ADIPOCYTES |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000265700100011 |
| 版本 | 出版稿 |
| 源URL | [http://202.127.25.143/handle/331003/1179]  |
| 专题 | 上海生化细胞研究所_上海生科院生化细胞研究所 |
| 推荐引用方式 GB/T 7714 | Yao, Y,Hong, SY,Zhou, H,et al. The differential protein and lipid compositions of noncaveolar lipid microdomains and caveolae[J]. CELL RESEARCH,2009,19(4):497-506. |
| APA | Yao, Y,Hong, SY,Zhou, H,Yuan, TC,Zeng, R,&Liao, K.(2009).The differential protein and lipid compositions of noncaveolar lipid microdomains and caveolae.CELL RESEARCH,19(4),497-506. |
| MLA | Yao, Y,et al."The differential protein and lipid compositions of noncaveolar lipid microdomains and caveolae".CELL RESEARCH 19.4(2009):497-506. |
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