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Function Study of EphA2 Gene in The Cell Apoptosis, Growth, Migration and Invasion of Glioma Cell Line U251
文献类型：期刊论文
| 作者 | Bai, YY; Shi, Y; Hui, GZ; Zhang, YR; Dong, WL; Hu, J; Jin, YX |
| 刊名 | PROGRESS IN BIOCHEMISTRY AND BIOPHYSICS
 |
| 出版日期 | 2009 |
| 卷号 | 36期号:4页码:464-470 |
| 关键词 | glioma U251 siRNA EphA2 |
| 通讯作者 | Bai, YY (reprint author), Soochow Univ, Dept Neurol, Affiliated Hosp 1, Suzhou 215006, Peoples R China.,dwlsz@163.com |
| 英文摘要 | In order to explore the function of EphA2 gene on the proliferation, apoptosis, motility and invasion of glioma cell line U251. The EphA2 mRNA levels of normal brain and two glioma cell lines U251 and U87 were detected by reverse transcription PCR (RT-PCR). Then, the function of EphA2 in glioma cell line U251 was analyzed by RNAi technology. The expression level of EphA2 gene was higher in U251 and U87 cells than that in normal brain. siRNA targeting EphA2 was designed and synthesized and then the siRNA was transfected into U251 cell with a high transfection efficiency of 70.31%. 50 nmol/L of siRNA could lead to over 70.0% reduction in EphA2 protein and mRNA level detected by Western blot and RT-PCR respectively. The cell proliferation was notably attenuated and the apoptosis was significantly increased after EhpA2 was downregulated. The migration and invasion of glioma cells were also attenuated when EhpA2 was knocked down by siRNA. EphA2 plays an important role in the malignance of glioma U251 cells and may be a novel molecular marker for the malignance of the glioma cell line U251. EphA2 could be a potential target in gene therapy for glioma. |
| 学科主题 | Biochemistry & Molecular Biology ; Biophysics |
| 类目[WOS] | Biochemistry & Molecular Biology ; Biophysics |
| 关键词[WOS] | PROTEIN-TYROSINE KINASES ; PANCREATIC ADENOCARCINOMA ; SIGNAL-TRANSDUCTION ; OVEREXPRESSION ; ACTIVATION ; GLIOBLASTOMA ; SUPPRESSES ; EXPRESSION ; RECEPTORS ; CARCINOMA |
| 收录类别 | SCI |
| 语种 | 中文 |
| WOS记录号 | WOS:000265411600011 |
| 版本 | 出版稿 |
| 源URL | [http://202.127.25.143/handle/331003/1182]  |
| 专题 | 上海生化细胞研究所_上海生科院生化细胞研究所 |
| 推荐引用方式 GB/T 7714 | Bai, YY,Shi, Y,Hui, GZ,et al. Function Study of EphA2 Gene in The Cell Apoptosis, Growth, Migration and Invasion of Glioma Cell Line U251[J]. PROGRESS IN BIOCHEMISTRY AND BIOPHYSICS,2009,36(4):464-470. |
| APA | Bai, YY.,Shi, Y.,Hui, GZ.,Zhang, YR.,Dong, WL.,...&Jin, YX.(2009).Function Study of EphA2 Gene in The Cell Apoptosis, Growth, Migration and Invasion of Glioma Cell Line U251.PROGRESS IN BIOCHEMISTRY AND BIOPHYSICS,36(4),464-470. |
| MLA | Bai, YY,et al."Function Study of EphA2 Gene in The Cell Apoptosis, Growth, Migration and Invasion of Glioma Cell Line U251".PROGRESS IN BIOCHEMISTRY AND BIOPHYSICS 36.4(2009):464-470. |
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