Crucial roles of MFZ-1 in the transcriptional regulation of apomorphine-induced modulation of FGF-2 expression in astrocytic cultures
文献类型：期刊论文
| 作者 | Luo, XY; Zhang, XH; Shao, W; Yin, YQ; Zhou, JW |
| 刊名 | JOURNAL OF NEUROCHEMISTRY
 |
| 出版日期 | 2009 |
| 卷号 | 108期号:4页码:952-961 |
| 关键词 | apomorphine astrocyte FGF-2 promoter gene regulation MZF-1 |
| 通讯作者 | Zhou, JW (reprint author), Chinese Acad Sci, Inst Neurosci, State Key Lab Neurobiol, Shanghai Inst Biol Sci, 320 Yueyang Rd, Shanghai 200031, Peoples R China.,jwzhou@ion.ac.cn |
| 英文摘要 | Apomorphine (APO), a potent D1/D2 dopamine receptor agonist, is used as an anti-parkinsonian drug. It stimulates the synthesis and release of multiple trophic factors in mesencephalic and striatal neurons, preventing the loss of dopaminergic neurons in vitro. Furthermore, APO enhances the biosynthesis and release of FGF-2 by activating dopamine receptors in striatal astrocytes, where cAMP/PKA and PKC/MAPK signalling cascades mediate this process. We investigate the effects of APO on the fibroblast growth factor-2 (FGF-2) promoter and its regulation in astrocytes and identify the transcription factor and cis element underlying these effects. In screening for cis-acting elements over the entire region of the FGF-2 promoter stimulated by APO in the astrocytes, a sequence located in the -785/-745 region was found to serve as the cis element. This element was recognized by the human myeloid zinc finger protein 1 (MZF-1) transcription factor. Introducing human MZF-1 plasmid and human MZF-1-specific siRNA has different effects on the FGF-2 promoter. Furthermore, it increases FGF-2 protein expression in HeLa cells and primary astrocytes, indicating that APO stimulates the FGF-2 promoter via the MZF-1 transcription factor. These data suggest that APO can enhance the biosynthesis and release of FGF-2 through the activation of the MZF-1 transcription factor in striatal astrocytes. |
| 学科主题 | Biochemistry & Molecular Biology; Neurosciences & Neurology |
| 类目[WOS] | Biochemistry & Molecular Biology ; Neurosciences |
| 关键词[WOS] | GROWTH FACTOR-2 EXPRESSION ; BLOOD-BRAIN-BARRIER ; PARKINSONS-DISEASE ; DOPAMINERGIC-NEURONS ; STRIATAL ASTROCYTES ; CARDIAC MYOCYTES ; PROMOTER REGION ; BASAL GANGLIA ; FACTOR GENE ; FIBROBLAST |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000262517100010 |
| 版本 | 出版稿 |
| 源URL | [http://202.127.25.143/handle/331003/1184]  |
| 专题 | 上海生化细胞研究所_上海生科院生化细胞研究所 |
| 推荐引用方式 GB/T 7714 | Luo, XY,Zhang, XH,Shao, W,et al. Crucial roles of MFZ-1 in the transcriptional regulation of apomorphine-induced modulation of FGF-2 expression in astrocytic cultures[J]. JOURNAL OF NEUROCHEMISTRY,2009,108(4):952-961. |
| APA | Luo, XY,Zhang, XH,Shao, W,Yin, YQ,&Zhou, JW.(2009).Crucial roles of MFZ-1 in the transcriptional regulation of apomorphine-induced modulation of FGF-2 expression in astrocytic cultures.JOURNAL OF NEUROCHEMISTRY,108(4),952-961. |
| MLA | Luo, XY,et al."Crucial roles of MFZ-1 in the transcriptional regulation of apomorphine-induced modulation of FGF-2 expression in astrocytic cultures".JOURNAL OF NEUROCHEMISTRY 108.4(2009):952-961. |
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